Linked Life Data

2937311

Source:http://linkedlifedata.com/resource/pubmed/id/2937311

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 2
pubmed:dateCreated
1986-4-18
pubmed:abstractText
Although synthetic atrial natriuretic peptides (ANP) have potent natriuretic/diuretic properties, the renal mechanisms underlying these effects are not yet clearly defined. To determine the possible sites of action for ANP within the kidney, we have localized 125I-ANP binding sites by in vitro autoradiography. Slices of rat kidney were incubated with 150 pM 125I-ANP [alpha-rat ANP (alpha-rANP), 28 amino acids] alone or in the presence of 10 nM or 1 microM unlabeled ANP. Autoradiography was performed using LKB Ultrofilm or emulsion-coated cover slips together with histochemical/immunohistochemical staining of specific tubular segments. High-affinity 125I-ANP binding sites were concentrated over glomeruli and to a lesser extent over the arterial vasculature. Lower-affinity binding sites were seen over proximal tubules and inner medullary collecting ducts. The remainder of the nephron was unlabeled. The localization of high-affinity 125I-ANP binding sites in rat kidney is consistent with a glomerular filtration/hemodynamic mechanism as the principal means by which ANP promotes natriuresis/diuresis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
250
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F573-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Localization of atrial natriuretic peptide binding sites within the rat kidney.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.