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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1980-3-17
|
| pubmed:abstractText |
We have recently demonstrated that normal hemopoietic cells express RNA sequences that are homologous to sequences specific for the Friend erythroleukemia virus genome [Bernstein, A., Gamble, C., Penrose, D. & Mak, T. W. (1979) Proc. Natl. Acad. Sci. USA 76, 4455-4459]. In this communication, we report that the Fv-2 locus, the major genetic determinant controlling host susceptibility to erythroleukemia induction by Friend leukemia virus, also controls the expression of endogenous sequences related to the replication-defective component of Friend leukemia virus, Friend spleen focus-forming virus (SFFV), in normal uninfected mice. Two independent congeneic pairs of mice [C57BL/6 (B6) and B6.S; B6 and B6.C(H-7(b))], differing only in a small region of the mouse genome including the Fv-2 locus, were used for this purpose. In both cases, molecular hybridization analysis indicated that the presence of SFFV-related RNA sequences in normal mice was associated with the Fv-2(s) allele: bone marrow or spleen cellular RNA from Fv-2(rr) B6 mice contained no detectable SFFV-related sequences, whereas their congeneic Fv-2(ss) pairs contained relatively high levels of these RNA sequences. The absence of these RNA sequences in Fv-2(rr) mice was not due to deletion of these sequences from the DNA of Fv-2(rr) mice. Repopulation of lethally irradiated Fv-2(rr) mice with syngeneic Fv-2(rr) bone marrow cells did not lead to any increase in the levels of these SFFV-related RNA sequences, suggesting that the expression of these sequences is still reduced or inhibited in actively cycling Fv-2(rr) hemopoietic cells. Infection with Friend leukemia virus resulted in the appearance of high levels of RNA homologous to SFFV-specific sequences in the leukemic spleens of B6.S (Fv-2(ss)) mice, whereas these cellular RNA sequences could not be detected in the spleens of Friend virus-infected B6 (Fv-2(rr)) mice. The demonstration that the same gene locus controls both the expression of exogenous SFFV-specific sequences and erythroleukemia induction by Friend leukemia virus suggests that these sequences may be necessary for erythroleukemic transformation. In addition, the finding that the Fv-2 gene locus controls the expression of endogenous SFFV-related sequences suggests that these sequences may also be involved in normal hemopoiesis.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-1087349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-1140862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-172913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-176368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-176594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-185623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-194058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-202189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-203404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-203405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-208270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-214581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-269439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-285212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-291976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-4325930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-4361674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-448285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-5317869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-5449211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-83198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/293685-902316
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0027-8424
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
76
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5809-12
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:293685-Animals,
pubmed-meshheading:293685-Bone Marrow,
pubmed-meshheading:293685-DNA, Viral,
pubmed-meshheading:293685-Defective Viruses,
pubmed-meshheading:293685-Friend murine leukemia virus,
pubmed-meshheading:293685-Genes, Viral,
pubmed-meshheading:293685-Hematopoietic Stem Cells,
pubmed-meshheading:293685-Mice,
pubmed-meshheading:293685-RNA, Viral,
pubmed-meshheading:293685-Spleen
|
| pubmed:year |
1979
|
| pubmed:articleTitle |
Fv-2 locus controls expression of Friend spleen focus-forming virus-specific sequences in normal and infected mice.
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| pubmed:publicationType |
Journal Article
|