Linked Life Data

2936651

Source:http://linkedlifedata.com/resource/pubmed/id/2936651

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-4-11
pubmed:abstractText
The lysis gene, E, of bacteriophage phi X174 has been subjected to deletion and gene fusion analysis. C-terminal deletions of as few as 17 of the 91 codons inactivate the cloned E gene, which in its intact form can cause lysis of the host cell. Fusion of lacZ to deletion joints at the 59th codon or beyond apparently restores lethal and lytic competence to the respective E deletion alleles, whereas a fusion at the 23rd codon remains non-lethal. The lethal E phi lacZ fusions are also lethal to a mutant, designated slyD, which was isolated as a spontaneous E. coli mutant resistant to the expression of the intact E gene. slyD appears to be linked to rpsE. The data are interpreted in terms of a model in which E-mediated lethality requires oligomerization of the E gene product. Calculations based on the beta-galactosidase activity accumulated by the time of lethal action of E phi lacZ suggest that fewer than 1000 molecules of E gene product are required for lysis and probably fewer than 100 are required for loss of host viability.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
39-46
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Deletion and fusion analysis of the phage phi X174 lysis gene E.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't