Linked Life Data

2934548

Source:http://linkedlifedata.com/resource/pubmed/id/2934548

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1986-2-17
pubmed:abstractText
Single fast myotomal fibres and small bundles of slow fibres (from the adductor pectoralis profundus muscle) were isolated from the Antarctic teleost Notothenia neglecta. Fibres were skinned by a brief detergent treatment. The effects of phosphate on the mechanical properties and ATPase activity of fast and slow fibres were studied. 20 mM-phosphate inhibited maximum isometric tension in slow fibres by 34%, but by only 11% in fast fibres. A half-maximal response was obtained at approximately 5 mM-phosphate. These concentrations are within the range measured in muscle, and the effect is probably of physiological significance. This species is of particular interest, since there is evidence that the energy supply to the fast muscle is largely based on phosphocreatine breakdown, which would result in large changes in intracellular phosphate concentration during exercise. The maximum contraction velocity of both fast and slow fibres was not affected by 10 mM-phosphate, nor was the ATPase activity of the slow fibres during isometric contraction. The phosphate-induced depression in tension in slow fibres was associated with a proportional decrease in stiffness. The rate of force recovery after rapid, small amplitude stretches and releases was increased by phosphate, as was the rate of rise of force during stretch activation. The results are discussed with reference to the different patterns of energy supply for contraction in muscle, and an attempt is made at explaining the data in terms of changes in cross-bridge kinetics.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-13485191, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-314510, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-3981474, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-4253003, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-4380573, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-4939977, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-5055791, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-5586931, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-6741702, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-6908844, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-6980967, http://linkedlifedata.com/resource/pubmed/commentcorrection/2934548-746545
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:volume
368
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
491-500
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Effects of phosphate on the contractile properties of fast and slow muscle fibres from an Antarctic fish.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't