Linked Life Data

2933156

Source:http://linkedlifedata.com/resource/pubmed/id/2933156

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1986-1-14
pubmed:abstractText
Following i.p. injection of [methyl-14C]methyl methanesulphonate (MMS) into rats (100 mg/kg) 3-[14C]methyladenine was identified as a urinary product excreted mainly up to 24 h after treatment, the amount over this period being about 0.02 mumol 3-methyladenine. When [14C]MMS and L-[methyl-3H]methionine were injected together no methyl-3H-label was detected in 3-methyladenine, nor was this product detected following injection of [methyl-14C]methionine alone or of [14C]formate. Isotopically labelled 1-methylnicotinamide (1-meNmd) was detected following all the treatments listed, and as previously found by Chu and Lawley, 1-meNmd excretion was enhanced by MMS treatment as judged by increased excretion of 1-[3H]meNmd when [14C]MMS and [3H]methionine were given together. The extent of labelling of 1-meNmd was much lower following injection of [14C] formate, than that from methionine or MMS. The results showed that 3-methyladenine derived only from direct chemical methylation by MMS. They also support the previous suggestion that [methyl-14C]meNmd can result from direct methylation, with a maximal amount of about 3% of excreted meNmd deriving from this route. The possible utility of the methods described for monitoring in vivo alkylation is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0009-2797
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
225-34
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Urinary excretion of 3-methyladenine and 1-methylnicotinamide by rats, following administration of [methyl-14C]methyl methanesulphonate and comparison with administration of [14C]methionine or formate.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't