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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1985-12-5
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pubmed:databankReference |
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pubmed:abstractText |
Apolipoprotein (apo) B-100 cDNAs were identified in a human liver cDNA library cloned in the expression vector lambda gt11. The beta-galactosidase-apoB-100 fusion protein was detected by two independently produced low density lipoprotein polyclonal antisera and by three apoB-100 monoclonal antibodies that crossreact with apoB-74. It was not recognized by two apoB-100 monoclonal antibodies that crossreact with apoB-26. The longest clone, lambda B8, was completely sequenced. It contains a 2.8-kilobase DNA fragment containing the codons for the carboxyl-terminal 836 amino acid residues of apo-B-100, as well as the 3' untranslated region of apoB-100 mRNA. We have thus mapped apoB-74 to the carboxyl-terminal portion of apoB-100. The deduced amino acid sequence of the cloned DNA matches the sequences of 14 apoB-100 peptides determined in our laboratory. Minor differences in amino acid sequence were noted in three of the peptides, suggesting polymorphism of apoB-100 at the protein and DNA levels. Secondary structure predictions reveal an unusual pattern for apolipoproteins, consisting of beta-structure (24%), alpha-helical content (33%), and random structure (30%). Ten amphipathic helical regions of 10-24 residues were identified. This carboxyl-terminal fragment of apoB-100 is considerably more hydrophobic than other apolipoproteins with known structure. Its lipid binding regions might include stretches of highly hydrophobic beta-sheets as well as amphipathic helices. Our findings on apoB structure might be important for understanding the role of apoB-100-containing lipoproteins in atherosclerosis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-13870229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-166603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-167825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-197883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-207346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-208540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-212203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-216702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-354496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-3923864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-4368333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-4369164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-5244750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6183259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6186232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6202688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6206837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6260957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6272870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6342527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6356359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6725258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6794632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-6930644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-7108955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-7110359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-7126555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-714156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-7229035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2932736-7263636
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7265-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2932736-Amino Acid Sequence,
pubmed-meshheading:2932736-Antibodies, Monoclonal,
pubmed-meshheading:2932736-Apolipoprotein B-100,
pubmed-meshheading:2932736-Apolipoproteins B,
pubmed-meshheading:2932736-Bacteriophage lambda,
pubmed-meshheading:2932736-Base Sequence,
pubmed-meshheading:2932736-Cloning, Molecular,
pubmed-meshheading:2932736-DNA,
pubmed-meshheading:2932736-DNA, Recombinant,
pubmed-meshheading:2932736-Lipoproteins, LDL,
pubmed-meshheading:2932736-Protein Conformation,
pubmed-meshheading:2932736-Recombinant Proteins
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pubmed:year |
1985
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pubmed:articleTitle |
Molecular cloning and expression of partial cDNAs and deduced amino acid sequence of a carboxyl-terminal fragment of human apolipoprotein B-100.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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