Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-4-21
pubmed:abstractText
Acrosin purified from an acidic extract of ejaculated goat spermatozoa migrated as a single 42,000-Mr band in SDS/polyacrylamide-gel electrophoresis. Reduction and alkylation of caprine acrosin produced two polypeptides, one of Mr 40,000 (heavy chain) and the other of Mr 3700 (light chain). The light chain purified by reversed-phase h.p.l.c. was a glycosylated octadecapeptide with an amino acid sequence similar to that of the N-terminal 18 residues of porcine acrosin light chain (78% positional identity). The sequence of the N-terminal 37 amino acids of purified caprine acrosin heavy chain is similar to that of porcine acrosin heavy chain (70% positional identity through 37 residues). Studies with synthetic substrates and synthetic and natural proteinase inhibitors confirmed both the specificity of the purified proteinase for Arg-Xaa and Lys-Xaa bonds and a serine-proteinase mechanism. Purified caprine acrosin hydrolysed the 90 kDa and 65 kDa components, but did not hydrolyse the 55 kDa component of the porcine zona pellucida. The action of the enzyme on the porcine zona pellucida was indistinguishable from that previously reported for porcine acrosin.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-115871, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-14389277, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-236308, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3113989, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3115322, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3480243, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3525830, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3548242, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3556269, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-359578, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3766967, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-383541, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3843705, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3882882, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3886029, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3888299, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-3894653, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-409339, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-4165, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-4826290, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-4980216, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-571894, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-6084, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-6231059, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-6378631, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-6411911, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-6818962, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-6855596, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-6947226, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-7007201, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-7007202, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-7040649, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-7043204, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-7066461, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-711760, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-736895, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-836299, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-901894, http://linkedlifedata.com/resource/pubmed/commentcorrection/2930460-986820
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
257
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
447-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Caprine acrosin. Purification, characterization and proteolysis of the porcine zona pellucida.
pubmed:affiliation
Department of Biochemistry and Biophysics, University of California, Davis 95616.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't