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rdf:type |
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lifeskim:mentions |
umls-concept:C0010028,
umls-concept:C0025255,
umls-concept:C0033684,
umls-concept:C0042523,
umls-concept:C0231491,
umls-concept:C0242768,
umls-concept:C0439799,
umls-concept:C0596235,
umls-concept:C0728938,
umls-concept:C1138842,
umls-concept:C1167622,
umls-concept:C1998793
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pubmed:issue |
1
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pubmed:dateCreated |
1989-4-6
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pubmed:abstractText |
A protein that binds the calcium-channel antagonist verapamil has been partially purified from maize (Zea mays) coleoptile membranes. The protein was solubilized with the detergent CHAPS ([ 3-(3-cholamidopropyl)dimethylammonio]propane-1-sulphonate) and purified by a combination of ion-exchange, gel-filtration and hydrophobic-interaction chromatography. This resulted in a 120-fold purification. SDS/polyacrylamide-gel-electrophoretic analysis of the polypeptides from the final purification step indicated that the verapamil-binding protein may have a major component of Mr 169,000. The dissociation constants for specific binding of [3H]verapamil to crude and CHAPS-solubilized maize coleoptile membrane fractions are 72 nM and 158 nM respectively, with respective binding-site concentrations of 135 pmol/mg of protein and 78 pmol/mg of protein. In both cases the Scatchard plots are linear, indicating a single class of binding sites. [3H]Verapamil binding to crude maize coleoptile membrane fractions could not be displaced by unlabelled desmethoxyverapamil or by nifedipine, but could be displaced by unlabelled methoxyverapamil.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-17747957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-2424495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-2435317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-2444223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-2997201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-3003067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-3034140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-3157687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-3753496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-4033782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6092067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6121350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6131381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6172996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6197906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6292744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6303205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6327306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-647023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-6618003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2920031-7070508
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
95-100
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
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pubmed:year |
1989
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pubmed:articleTitle |
Partial purification of a protein from maize (Zea mays) coleoptile membranes binding the Ca2+-channel antagonist verapamil.
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pubmed:affiliation |
Department of Botany, University of Edinburgh, Scotland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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