Linked Life Data

2917524

Source:http://linkedlifedata.com/resource/pubmed/id/2917524

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-4-3
pubmed:abstractText
Two forms of glycosylated PRL (G-PRL) which differed in their binding properties to Concanavalin-A (Con-A) were isolated from human pituitary glands. One form, G1-hPRL, was only slightly retarded by Con-A; the other, G2-hPRL, was adsorbed by Con-A and could be eluted with methyl-D-manno-pyranoside, an indication of differing carbohydrate units in the two G-PRLs. Differences in type of glycosylation were also indicated by HPLC peptide mapping of tryptic digests of the two forms. The elution time for the tryptic peptide carrying the asparagine-linked carbohydrate unit varied for the two G-PRLs. The results point to the asparagine at position 31 as being the site of attachment of the carbohydrate. The carbohydrate structure influenced the crop sac-stimulating activity of the G-hPRLs. G1-hPRL had only about one fourth the activity of the reference standard (nonglycosylated ovine PRL, 35 IU/mg). The form that bound to Con-A, G2-hPRL, was equipotent to the reference standard. Because glycosylated forms have varying biological activities and are major components of circulating PRL, the physiological significance of serum concentrations of PRL measured by RIA will have to be reevaluated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1558-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Two forms of glycosylated human prolactin have different pigeon crop sac-stimulating activities.
pubmed:affiliation
Lutcher Brown Department of Biochemistry, Whittier Institute for Diabetes and Endocrinology, La Jolla, California 92037.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.