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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1989-3-13
|
| pubmed:abstractText |
The biosynthesis and processing of the human mannose receptor has been studied in monocyte-derived macrophages. Adherent cells were labeled for 60 min with Trans35S (a mixture of 35S-labeled methionine and cysteine), chased, and subjected to immunoprecipitation by antibody raised against the human placental receptor. The antibody immunoprecipitated a single protein of molecular mass 162 kDa; precipitation of the labeled receptor could be inhibited by placental receptor. The results presented demonstrate that the receptor is synthesized as a 154-kDa precursor which is processed to 162 kDa in 90 min. The precursor is a glycoprotein bearing endoglycosidase H-sensitive oligosaccharides; the 162-kDa form is endoglycosidase H-resistant but peptide:N-glycanase-sensitive. Desialylation of the mannose receptor with neuraminidase generates a protein which is recognized by peanut agglutinin, a lectin that specifically binds desialylated O-linked oligosaccharides. Thus, the human macrophage mannose receptor bears both N- and O-linked oligosaccharide chains. Newly synthesized mannose receptor exhibits a half-life of 33 h as determined by pulse-chase studies. This indicates that on the average, each molecule of receptor recycles between the cell surface and endosomes hundreds of times before degradation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose-Binding Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/mannose receptor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2385-90
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2914913-Antibodies,
pubmed-meshheading:2914913-Antibody Specificity,
pubmed-meshheading:2914913-Cells, Cultured,
pubmed-meshheading:2914913-Female,
pubmed-meshheading:2914913-Glycoproteins,
pubmed-meshheading:2914913-Humans,
pubmed-meshheading:2914913-Kinetics,
pubmed-meshheading:2914913-Lectins, C-Type,
pubmed-meshheading:2914913-Macrophages,
pubmed-meshheading:2914913-Mannose,
pubmed-meshheading:2914913-Mannose-Binding Lectins,
pubmed-meshheading:2914913-Molecular Weight,
pubmed-meshheading:2914913-Oligosaccharides,
pubmed-meshheading:2914913-Placenta,
pubmed-meshheading:2914913-Pregnancy,
pubmed-meshheading:2914913-Protein Processing, Post-Translational,
pubmed-meshheading:2914913-Receptors, Cell Surface,
pubmed-meshheading:2914913-Receptors, Immunologic
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Biosynthesis and processing of the mannose receptor in human macrophages.
|
| pubmed:affiliation |
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|