Linked Life Data

2914013

Source:http://linkedlifedata.com/resource/pubmed/id/2914013

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-2-28
pubmed:abstractText
Chinese hamster ovary cells were exposed to various concentrations of diethylmaleate in order to produce various levels of intracellular glutathione (GSH) depletion. Exposure to a 20 microM concentration or more of diethylmaleate depleted the intracellular glutathione concentration by 80% or more and resulted in enhanced synthesis of two 32 kDa proteins which exhibited a pI of about 6.5. Exposure of cells to 50 microM buthionine sulfoximine for 24 hr reduced GSH levels by 95% but did not enhance the synthesis of this protein. Addition of diethylmaleate to buthionine sulfoximine-treated cells resulted in enhanced synthesis of the 32 kDa protein however. Exposure to 0.4 mM diamide triggered the synthesis of several heat shock proteins but did not induce the synthesis of the 32 kDa protein. These results indicated that enhanced synthesis of the 32 kDa protein occurred only after glutathione depletion exceeded 80% and required formation of a glutathione conjugate.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
299-304
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Glutathione conjugation and induction of a 32,000 dalton stress protein.
pubmed:affiliation
Vanderbilt Center for Radiation Oncology, Vanderbilt University School of Medicine, Nashville, TN 37232.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.