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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-2-21
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pubmed:abstractText |
Addition of ATP to a highly purified fraction of rat liver nuclei incubated with submicromolar concentrations of Ca2+ and trace amounts of 45Ca2+ resulted in the rapid accumulation of 45Ca2+ in the nuclei. This was associated with an increase in intranuclear free Ca2+ concentration as measured with the fluorescent dye 1-[2-(5-carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-a mino-5'- methylphenoxy)ethane-N,N,N',N'-tetraacetic acid (fura-2). Inhibitors of microsomal and mitochondrial Ca2+ translocases had no effect on nuclear Ca2+ sequestration, indicating that it was distinct from previously known intracellular Ca2+-transporting systems. Ca2+ uptake and the associated increase in intranuclear free Ca2+ concentration were prevented by calmidazolium, a potent calmodulin antagonist. Partial characterization of the ATP-stimulated nuclear Ca2+ uptake showed that maximal rates of Ca2+ uptake and increase in intranuclear free Ca2+ level occurred at concentrations of Ca2+ normally present in the cytosol of mammalian cells. Together, these results show that a distinct, ATP- and calmodulin-dependent Ca2+ uptake system exists in liver nuclei. This system may play an important role in the regulation of intranuclear Ca2+-dependent processes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-2822084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-2931050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-2961610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-3103219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-3127728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-3369679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-3611090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-3680375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-3838803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-3934562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-4250976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-6116712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-6134732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-6146314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-6203171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-6214287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-6272758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-6987650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2911591-7130192
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
453-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2911591-Adenosine Triphosphate,
pubmed-meshheading:2911591-Animals,
pubmed-meshheading:2911591-Biological Transport, Active,
pubmed-meshheading:2911591-Calcium,
pubmed-meshheading:2911591-Calmodulin,
pubmed-meshheading:2911591-Cell Nucleus,
pubmed-meshheading:2911591-Imidazoles,
pubmed-meshheading:2911591-Liver,
pubmed-meshheading:2911591-Male,
pubmed-meshheading:2911591-Microscopy, Fluorescence,
pubmed-meshheading:2911591-Rats,
pubmed-meshheading:2911591-Rats, Inbred Strains
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pubmed:year |
1989
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pubmed:articleTitle |
ATP stimulates Ca2+ uptake and increases the free Ca2+ concentration in isolated rat liver nuclei.
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pubmed:affiliation |
Department of Toxicology, Karolinska Institutet, Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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