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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1989-3-23
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pubmed:abstractText |
A new method has been described for removing a very small number of contaminating astrocytes in neuronal cultures (derived from the septal-diagonal band region of 17-day-old embryonic rat brain) grown in a chemically defined medium. The proportion of these glial fibrillary acidic protein (GFAP)-positive cells was usually less than 1.5% up to 10 days, but thereafter their number increased rapidly reaching 10-15% by 22 days in vitro. A prolonged exposure to normally used concentration of cytosine arabinoside (Ara-C; 10 microM) was toxic to both astroglial and neuronal cells, while a brief treatment (48 h) with a low level (4 microM) of Ara-C failed to eliminate these astrocytes, as judged by glutamine synthetase activity and GFAP-positive cell count. However, these quiescent astroglial cells could be easily eliminated if they were induced to proliferate by epidermal growth factor before exposure to Ara-C. The combined treatment with these agents had no effect on the number of acetylcholinesterase-positive cells, and on the development of cholinergic and GABA-ergic neurons, as measured in terms of choline acetyltransferase and glutamate decarboxylase activity, respectively.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Cytarabine,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate-Ammonia Ligase,
http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-8993
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
470
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
283-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2905925-Animals,
pubmed-meshheading:2905925-Astrocytes,
pubmed-meshheading:2905925-Brain,
pubmed-meshheading:2905925-Cell Separation,
pubmed-meshheading:2905925-Cell Survival,
pubmed-meshheading:2905925-Cells, Cultured,
pubmed-meshheading:2905925-Culture Media,
pubmed-meshheading:2905925-Cytarabine,
pubmed-meshheading:2905925-Embryo, Mammalian,
pubmed-meshheading:2905925-Epidermal Growth Factor,
pubmed-meshheading:2905925-Glial Fibrillary Acidic Protein,
pubmed-meshheading:2905925-Glutamate-Ammonia Ligase,
pubmed-meshheading:2905925-L-Lactate Dehydrogenase,
pubmed-meshheading:2905925-Neurons,
pubmed-meshheading:2905925-Rats
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pubmed:year |
1988
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pubmed:articleTitle |
A novel way of removing quiescent astrocytes in a culture of subcortical neurons grown in a chemically defined medium.
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pubmed:affiliation |
MRC Developmental Neurobiology Unit, London, U.K.
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pubmed:publicationType |
Journal Article
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