Linked Life Data

2903862

Source:http://linkedlifedata.com/resource/pubmed/id/2903862

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
34
pubmed:dateCreated
1989-1-3
pubmed:abstractText
Through substitution mutagenesis and gene transfer experiments in cultured cells, we have identified three sequences in the 5' flanking region of the gene for hamster 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase that are required for sterol-mediated regulation of transcription. Point mutations in any one of these sequences largely prevented the increase in transcription that normally follows cellular sterol depletion. These mutations did not alter the low level of transcription that occurs in the presence of sterols. Two of the three sterol regulatory sequences contain an octanucleotide that shows a 7/8-base pair match with a sequence that was previously identified as a sterol regulatory element in the genes for HMG-CoA reductase and the low density lipoprotein receptor, both of which are induced by sterol deprivation. The third sterol regulatory region in the HMG-CoA synthase promoter shows only a low-level match with the other sterol regulatory elements. The current data suggest that the sterol regulatory elements in the HMG-CoA synthase promoter operate by a conditional positive mechanism: in the absence of sterols, regulatory proteins bind to these elements and stimulate transcription; in the presence of sterols, the regulatory proteins are inactivated and transcription decreases to the basal rate.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18480-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Multiple sterol regulatory elements in promoter for hamster 3-hydroxy-3-methylglutaryl-coenzyme A synthase.
pubmed:affiliation
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't