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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-12-1
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pubmed:abstractText |
A high-performance strong cation-exchange Sulfoethyl Aspartamide column was used to analyze and purify five N-terminal pyroglutamyl peptides after treatment with Pyroglutamate Aminopeptidase. The resulting deblocked N-1 peptides possess an increased positive charge and are therefore retained to a greater extent by the column. Salt gradient elution in a pH 3 mobile phase was then used to recover the desired peptides and the purified deblocked peptides were directly subjected to N-terminal sequence analysis. The same digests were also chromatographed on a C18 reversed-phase column using standard trifluoroacetic acid-acetonitrile gradient elution. The elution order for the parent peptide and the N-1 peptide on the reversed-phase column was reversed from that on the Sulfoethyl Aspartamide column and the resolution of the two peptides obtained on the reversed-phase column was less than that observed on the cation-exchange column. In addition, the Sulfoethyl Aspartamide column was shown to be useful to monitor the extent of N-terminal glutamine cyclization formed during peptide purification and storage.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkanesulfonates,
http://linkedlifedata.com/resource/pubmed/chemical/Aminopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensinogen,
http://linkedlifedata.com/resource/pubmed/chemical/Bombesin,
http://linkedlifedata.com/resource/pubmed/chemical/Diazepam Binding Inhibitor,
http://linkedlifedata.com/resource/pubmed/chemical/Eledoisin,
http://linkedlifedata.com/resource/pubmed/chemical/Gonadotropin-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pyroglutamyl-Peptidase I,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidonecarboxylic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Silicon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Teprotide,
http://linkedlifedata.com/resource/pubmed/chemical/Thymic Factor, Circulating,
http://linkedlifedata.com/resource/pubmed/chemical/diazepam binding inhibitor (33-50),
http://linkedlifedata.com/resource/pubmed/chemical/poly(2-sulfoethyl aspartamide)silica
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
156
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
910-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2903737-Alkanesulfonates,
pubmed-meshheading:2903737-Aminopeptidases,
pubmed-meshheading:2903737-Angiotensinogen,
pubmed-meshheading:2903737-Bombesin,
pubmed-meshheading:2903737-Chromatography, High Pressure Liquid,
pubmed-meshheading:2903737-Diazepam Binding Inhibitor,
pubmed-meshheading:2903737-Eledoisin,
pubmed-meshheading:2903737-Gonadotropin-Releasing Hormone,
pubmed-meshheading:2903737-Neuropeptides,
pubmed-meshheading:2903737-Neurotensin,
pubmed-meshheading:2903737-Oligopeptides,
pubmed-meshheading:2903737-Peptide Fragments,
pubmed-meshheading:2903737-Peptides,
pubmed-meshheading:2903737-Pyroglutamyl-Peptidase I,
pubmed-meshheading:2903737-Pyrrolidinones,
pubmed-meshheading:2903737-Pyrrolidonecarboxylic Acid,
pubmed-meshheading:2903737-Receptors, Interleukin-2,
pubmed-meshheading:2903737-Silicon Dioxide,
pubmed-meshheading:2903737-Teprotide,
pubmed-meshheading:2903737-Thymic Factor, Circulating
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pubmed:year |
1988
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pubmed:articleTitle |
Facile analysis and purification of deblocked N-terminal pyroglutamyl peptides with a strong cation-exchange sulfoethyl aspartamide column.
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pubmed:affiliation |
Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110.
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pubmed:publicationType |
Journal Article
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