Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1988-12-9
pubmed:abstractText
The protein encoded by the neu protooncogene (human gene symbol NGL for neuro/glioblastoma-derived) is a member of the surface receptor/tyrosine kinase family. Though its structure suggests that it can transduce a transmembrane signal, neither its extracellular ligand nor its critical intracellular substrates are known. To explore the functional properties of the protein encoded by neu, we created a fusion gene that joins the cytoplasmic domain of neu to the extracellular portion of an immunoglobulin heavy chain. The localization of the fusion polypeptide can then be controlled by coexpression with immunoglobulin light chain. In the absence of light chain, the heavy chain-neu polypeptide is expressed intracellularly and has no transforming activity. By contrast, in the presence of light chain the fusion polypeptide is expressed at the cell surface and produces tumorigenic foci. Thus, transformation apparently requires expression at the cell surface, where the neu intracellular domain can interact with components that are localized to the plasma membrane. The fusion protein is active in cellular transformation when the transmembrane domain is derived either from neu or from immunoglobulin, indicating that the neu transmembrane domain is not specifically required for transformation, although neu activation in tumors is known to result from a point mutation in this region. The extracellular immunoglobulin heavy and light chain domains of the fusion protein form a functional binding site that allows antigen to modulate its activity, reversing the transforming effect.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-222468, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-2860972, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-2869410, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-2871941, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-2878363, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-2885917, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-2992089, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-2992090, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3008004, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3011032, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3018522, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3024008, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3027579, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3032456, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3039909, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3104909, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3460176, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3798106, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3923101, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3935328, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3936040, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-3945311, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6112749, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6286831, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6296087, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6308472, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6320011, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6330080, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6335048, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6390182, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6775818, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-6788890, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903500-7003017
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8057-61
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
neu protooncogene fused to an immunoglobulin heavy chain gene requires immunoglobulin light chain for cell surface expression and oncogenic transformation.
pubmed:affiliation
Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't