Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1988-12-20
pubmed:abstractText
Cells of Bordetella pertussis BP353, a nonfimbriated Eldering serotype 1.3 strain, were used as an immunogen to produce three monoclonal antibodies, BPE3, BPD8, and BPE8, that agglutinated the immunizing cells, as well as certain other nonfimbriated and fimbriated serotype 3-containing B. pertussis strains. The antibodies did not agglutinate serotype 1 or nontypable B. pertussis cells. These monoclonal antibodies specifically detected a 69-kilodalton (kDa) band on Western blots (immunoblots) containing whole B. pertussis cell lysates of Eldering agglutinogen serotypes 1.3, 1.3.6, 1.2.3.4, and 1.2.3.4.6. This 69-kDa antigen was released from the bacteria by cell incubation for 60 min at 60 degrees C, and it was purified by affinity chromatography with a BPE3-agarose affinity matrix. Purified material was used to produce a polyclonal antiserum that agglutinated all nonfimbriated and fimbriated B. pertussis cells containing serotype 3 agglutinogen. Immunogold electron microscopy and indirect immunofluorescence studies demonstrated that it is an outer membrane constituent but nonfimbrial in appearance. BPE3 did not detect purified fimbriae on Western blots, and antibodies to these fimbriae did not bind to the 69-kDa component. Although B. bronchiseptica and B. parapertussis cells were not agglutinated by the monoclonal antibodies, antigenically similar proteins were detected in extracts of the bacteria. These results identify the 69-kDa protein as a nonfimbrial agglutinogen present on all virulent strains of B. pertussis. The monoclonal antibodies described here should be useful for further studies on the structure and function of this protein.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-13123910, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-13475208, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2859248, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2872097, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2872101, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2872103, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2872113, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2875062, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2875949, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2881893, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2883653, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2885755, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2893776, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2903125, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2907088, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-2931379, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-3317732, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-3972452, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-4044033, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-4312923, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-4370715, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-5941389, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-6127315, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-6175245, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-6276465, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-6311749, http://linkedlifedata.com/resource/pubmed/commentcorrection/2903126-7009747
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3189-95
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Identification of a 69-kilodalton nonfimbrial protein as an agglutinogen of Bordetella pertussis.
pubmed:affiliation
Division of Bacterial Products, Food and Drug Administration, Bethesda, Maryland.
pubmed:publicationType
Journal Article