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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1988-12-6
|
| pubmed:databankReference | |
| pubmed:abstractText |
Growth hormone factor 1 (GHF-1) is a pituitary-specific transcription factor that plays a critical role in cell type-specific expression of the growth hormone (GH) gene. Here, we describe the isolation of bovine and rat GHF-1 cDNA clones. These cDNAs encode proteins whose molecular mass, 33K, is identical to purified GHF-1 and whose sequence agrees with a partial GHF-1 peptide sequence. The predicted GHF-1 sequence contains a region, near its C-terminus, that exhibits considerable similarity to a homeobox consensus sequence. DNAase I footprinting with bacterially expressed fusion protein containing a fragment of GHF-1 encompassing the homeobox indicates that this region of the protein functions as its DNA binding domain. Expression of GHF-1 is restricted to cells of the somatotropic lineage in the pituitary. This remarkable specificity of GHF-1 expression correlates with the selective transcription of its target, the GH gene. Other mammalian homeobox-containing proteins may function similarly as transcription factors controlling cell type-specific expression in other locations.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0092-8674
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
4
|
| pubmed:volume |
55
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
505-18
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2902927-Amino Acid Sequence,
pubmed-meshheading:2902927-Animals,
pubmed-meshheading:2902927-Base Sequence,
pubmed-meshheading:2902927-Cattle,
pubmed-meshheading:2902927-Chromatography, Affinity,
pubmed-meshheading:2902927-DNA,
pubmed-meshheading:2902927-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2902927-Female,
pubmed-meshheading:2902927-Fluorescent Antibody Technique,
pubmed-meshheading:2902927-Gene Expression Regulation,
pubmed-meshheading:2902927-Genes, Homeobox,
pubmed-meshheading:2902927-Growth Hormone,
pubmed-meshheading:2902927-Molecular Sequence Data,
pubmed-meshheading:2902927-Peptides,
pubmed-meshheading:2902927-Pituitary Gland,
pubmed-meshheading:2902927-Rats,
pubmed-meshheading:2902927-Transcription, Genetic,
pubmed-meshheading:2902927-Transcription Factors
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein.
|
| pubmed:affiliation |
Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|