Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1988-11-3
|
| pubmed:abstractText |
We have evaluated the morphology, function, and TCR mRNA and DNA profiles of Thy-1+ epidermal cells (EC) derived from athymic (nude) mice. Based on these criteria we demonstrate that Thy-1+ EC in nude mice are predominantly round and angular and located in follicular epithelium, as compared to predominantly dendritic, interfollicular Thy-1+ EC found in normal CBA or BALB/c mice. Functionally, Thy-1+ EC from normal mice proliferate and elaborate IL-2 in response to stimulation by Con A; by contrast, nude Thy-1+ EC fail to proliferate or secrete IL-2 in response to Con A. Nude Thy-1+ EC proliferate markedly in response to low-dose IL-2 alone; Thy-1+ EC from normal mice give only modest proliferative responses to IL-2. Both populations of cells proliferate and elaborate IL-2 in response to PMA and calcium ionophore. Short-term cultured Thy-1+ EC from nude mice resemble Thy-1+ EC from normal animals in that they express no detectable functional TCR-alpha and beta-RNA. Normal Thy-1+ EC express abundant levels of functional RNA for TCR-gamma and delta-chains and for the CD3 delta-chain, whereas nude Thy-1+ EC produce no detectable TCR-delta and no CD3 delta-RNA and only truncated, presumably germ-line TCR-gamma transcripts, as suggested by lack of hybridization with gamma-V region probes and by lack of detectable rearrangements in the gamma-genes. These phenotypic, functional, and genotypic characteristics of Thy-1+ EC from nude mice suggest that these cells are at a very early stage of T cell differentiation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Ethers,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
141
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1897-903
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2902136-Animals,
pubmed-meshheading:2902136-Antigens, Surface,
pubmed-meshheading:2902136-Antigens, Thy-1,
pubmed-meshheading:2902136-Blotting, Northern,
pubmed-meshheading:2902136-Blotting, Southern,
pubmed-meshheading:2902136-Cell Division,
pubmed-meshheading:2902136-Concanavalin A,
pubmed-meshheading:2902136-Dendritic Cells,
pubmed-meshheading:2902136-Epidermis,
pubmed-meshheading:2902136-Ethers,
pubmed-meshheading:2902136-Female,
pubmed-meshheading:2902136-Interleukin-2,
pubmed-meshheading:2902136-Ionomycin,
pubmed-meshheading:2902136-Male,
pubmed-meshheading:2902136-Mice,
pubmed-meshheading:2902136-Mice, Inbred BALB C,
pubmed-meshheading:2902136-Mice, Inbred CBA,
pubmed-meshheading:2902136-Mice, Nude,
pubmed-meshheading:2902136-Receptors, Antigen, T-Cell,
pubmed-meshheading:2902136-Species Specificity,
pubmed-meshheading:2902136-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2902136-Thymus Gland
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice. A study of morphology, function, and T cell receptor expression.
|
| pubmed:affiliation |
Department of Dermatology, University of Texas Health Science Center, Dallas 75235.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|