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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-10-17
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pubmed:abstractText |
The rat spinal cord slice preparation has been used to investigate the sensitivity of deep dorsal horn neurones to the excitatory amino acids N-methyl-D-aspartate (NMDA), quisqualate and L-glutamate. Intracellular recordings were made from 44 neurones in laminae III-VI of 14- to 16-day rats. Superfusion of quisqualate (30 microM) excited all neurones, NMDA (50 microM) excited 72% and L-glutamate (0.5-1 mM) 63% of the neurones. Depolarizations were retained after tetrodotoxin but with a reduced amplitude. The NMDA antagonist D-aminophosphonovalerate (D-APV, 10 microM) reduced NMDA and L-glutamate depolarizations by 66% and by 40%, respectively, while the quisqualate responses were enhanced by 27%. Dorsal root stimulation elicited two main patterns of activity; short-latency single/double spikes followed by subthreshold excitatory postsynaptic potentials (EPSPs) or a burst of spikes rising from a long duration composite EPSP. D-APV reduced the long-latency components of the first type and reduced the amplitude and duration of the composite EPSP of the second. These results support a specialized role for NMDA receptors in synaptic transmission in the dorsal horn.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0304-3940
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
286-92
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pubmed:dateRevised |
2009-9-29
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pubmed:meshHeading |
pubmed-meshheading:2901700-2-Amino-5-phosphonovalerate,
pubmed-meshheading:2901700-Amino Acids,
pubmed-meshheading:2901700-Animals,
pubmed-meshheading:2901700-Aspartic Acid,
pubmed-meshheading:2901700-Electrophysiology,
pubmed-meshheading:2901700-Glutamates,
pubmed-meshheading:2901700-Glutamic Acid,
pubmed-meshheading:2901700-Intracellular Membranes,
pubmed-meshheading:2901700-N-Methylaspartate,
pubmed-meshheading:2901700-Oxadiazoles,
pubmed-meshheading:2901700-Quisqualic Acid,
pubmed-meshheading:2901700-Rats,
pubmed-meshheading:2901700-Spinal Cord,
pubmed-meshheading:2901700-Tetrodotoxin,
pubmed-meshheading:2901700-Valine
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pubmed:year |
1988
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pubmed:articleTitle |
An intracellular analysis of amino acid induced excitations of deep dorsal horn neurones in the rat spinal cord slice.
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pubmed:affiliation |
Department of Anatomy, University College London, U.K.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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