Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1988-9-20
|
| pubmed:abstractText |
Serial transplantation and competitive repopulation were used to evaluate any loss of self-replicative capacity of bone marrow stem cells in a mouse model with increased and persistent hemopoietic demands. Congenic marrows from old control and from young and old mice with hereditary spherocytic anemia (sphha/sphha) were serially transplanted at 35-day intervals into normal irradiated recipients. Old anemic marrow failed or reverted to recipient karyotype at a mean of 3.5 transplants, and young anemic marrow reverted at a mean of 4.0 transplants, whereas controls did so at a mean of 5.0 transplants. In a competitive assay in which a mixture of anemic and control marrow was transplanted, the anemic marrow persisted to 10 months following transplantation; anemic marrow repopulation was greater if anemic marrow sex matched with the host. It is possible that lifelong stress of severe anemia decreases stem cell reserve in the anemic sphha/sphha mouse marrow. However, marginal differences in serial transplantation number and the maintenance of anemic marrow in a competition assay would suggest that marrow stem cells, under prolonged stress, are capable of exhibiting good repopulating and self-replicating abilities.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0301-472X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
653-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2900155-Aging,
pubmed-meshheading:2900155-Animals,
pubmed-meshheading:2900155-Bone Marrow,
pubmed-meshheading:2900155-Bone Marrow Transplantation,
pubmed-meshheading:2900155-Cell Cycle,
pubmed-meshheading:2900155-Chronic Disease,
pubmed-meshheading:2900155-Colony-Forming Units Assay,
pubmed-meshheading:2900155-Disease Models, Animal,
pubmed-meshheading:2900155-Erythrocyte Count,
pubmed-meshheading:2900155-Female,
pubmed-meshheading:2900155-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:2900155-Hematopoietic Stem Cells,
pubmed-meshheading:2900155-Male,
pubmed-meshheading:2900155-Mice,
pubmed-meshheading:2900155-Mice, Inbred C57BL,
pubmed-meshheading:2900155-Mice, Mutant Strains,
pubmed-meshheading:2900155-Pancytopenia,
pubmed-meshheading:2900155-Radiation Chimera,
pubmed-meshheading:2900155-Spherocytosis, Hereditary,
pubmed-meshheading:2900155-Spleen
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Evaluation of stem cell reserve using serial bone marrow transplantation and competitive repopulation in a murine model of chronic hemolytic anemia.
|
| pubmed:affiliation |
Department of Pathology, University of Washington School of Medicine, Seattle 98195.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|