pubmed-article:2899552 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0012634 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0031921 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0243040 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0444749 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0018494 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0033414 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0205224 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
pubmed-article:2899552 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
pubmed-article:2899552 | pubmed:issue | 8 | lld:pubmed |
pubmed-article:2899552 | pubmed:dateCreated | 1988-8-31 | lld:pubmed |
pubmed-article:2899552 | pubmed:abstractText | RDEC-1, an Escherichia coli strain that adheres to rabbit mucosa and causes an attaching, effacing lesion, expresses the pilus adhesin AF/R1 which determines in vitro attachment to rabbit intestinal brush borders. In order to determine the role of AF/R1 pili in the pathogenesis of enteropathogenic diarrhea in rabbits, we localized the genes for AF/R1 expression, constructed an AF/R1- strain, and compared the virulence of the AF/R1+ and AF/R1- strains with particular attention to the development of attaching, effacing lesions. We introduced Tn5 into the 86-megadalton (MDa) conjugative plasmid known to mediate expression of AF/R1 pili and transferred the derivative plasmids into laboratory strain HB101. Transconjugant M5 was found to contain the 86-MDa plasmid from RDEC-1 and to express AF/R1 pili. Pilus expression on M5 was confirmed by reaction with antiserum raised against purified AF/R1 pili and allowed the bacteria to adhere to the rabbit ileum in an in vitro assay. Three Tn5 insertions in the 86-MDa plasmid were obtained which resulted in loss of AF/R1 expression. Part of the plasmid was mapped, including a region necessary for AF/R1 pilus expression. AF/R1- mutant strain M34 was constructed, and its pathogenesis was investigated. M34 produced disease in rabbits but was less virulent than the parent. The characteristic effacing lesions of RDEC-1 and enteropathogenic E. coli developed in the intestine of rabbits infected with either M34 or RDEC-1, although with M34 they were much less frequent and did not involve the small bowel. We conclude that AF/R1 pilus expression is not essential for the attaching, effacing lesion but serves as an accessory virulence factor which promotes an initial interaction of RDEC-1 with normal epithelial cells. | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:language | eng | lld:pubmed |
pubmed-article:2899552 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:2899552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2899552 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2899552 | pubmed:month | Aug | lld:pubmed |
pubmed-article:2899552 | pubmed:issn | 0019-9567 | lld:pubmed |
pubmed-article:2899552 | pubmed:author | pubmed-author:BoedekerE CEC | lld:pubmed |
pubmed-article:2899552 | pubmed:author | pubmed-author:AndrewsG PGP | lld:pubmed |
pubmed-article:2899552 | pubmed:author | pubmed-author:WolfM KMK | lld:pubmed |
pubmed-article:2899552 | pubmed:author | pubmed-author:FritzD LDL | lld:pubmed |
pubmed-article:2899552 | pubmed:author | pubmed-author:SjogrenR... | lld:pubmed |
pubmed-article:2899552 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2899552 | pubmed:volume | 56 | lld:pubmed |
pubmed-article:2899552 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2899552 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2899552 | pubmed:pagination | 1846-57 | lld:pubmed |
pubmed-article:2899552 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:meshHeading | pubmed-meshheading:2899552-... | lld:pubmed |
pubmed-article:2899552 | pubmed:year | 1988 | lld:pubmed |
pubmed-article:2899552 | pubmed:articleTitle | Characterization of the plasmid from Escherichia coli RDEC-1 that mediates expression of adhesin AF/R1 and evidence that AF/R1 pili promote but are not essential for enteropathogenic disease. | lld:pubmed |
pubmed-article:2899552 | pubmed:affiliation | Department of Gastroenterology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100. | lld:pubmed |
pubmed-article:2899552 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:2899552 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2899552 | lld:pubmed |