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rdf:type |
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lifeskim:mentions |
umls-concept:C0012634,
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0018494,
umls-concept:C0031921,
umls-concept:C0032136,
umls-concept:C0033414,
umls-concept:C0086597,
umls-concept:C0185117,
umls-concept:C0205224,
umls-concept:C0243040,
umls-concept:C0332120,
umls-concept:C0444749,
umls-concept:C1880022,
umls-concept:C2911684
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pubmed:issue |
8
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pubmed:dateCreated |
1988-8-31
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pubmed:abstractText |
RDEC-1, an Escherichia coli strain that adheres to rabbit mucosa and causes an attaching, effacing lesion, expresses the pilus adhesin AF/R1 which determines in vitro attachment to rabbit intestinal brush borders. In order to determine the role of AF/R1 pili in the pathogenesis of enteropathogenic diarrhea in rabbits, we localized the genes for AF/R1 expression, constructed an AF/R1- strain, and compared the virulence of the AF/R1+ and AF/R1- strains with particular attention to the development of attaching, effacing lesions. We introduced Tn5 into the 86-megadalton (MDa) conjugative plasmid known to mediate expression of AF/R1 pili and transferred the derivative plasmids into laboratory strain HB101. Transconjugant M5 was found to contain the 86-MDa plasmid from RDEC-1 and to express AF/R1 pili. Pilus expression on M5 was confirmed by reaction with antiserum raised against purified AF/R1 pili and allowed the bacteria to adhere to the rabbit ileum in an in vitro assay. Three Tn5 insertions in the 86-MDa plasmid were obtained which resulted in loss of AF/R1 expression. Part of the plasmid was mapped, including a region necessary for AF/R1 pilus expression. AF/R1- mutant strain M34 was constructed, and its pathogenesis was investigated. M34 produced disease in rabbits but was less virulent than the parent. The characteristic effacing lesions of RDEC-1 and enteropathogenic E. coli developed in the intestine of rabbits infected with either M34 or RDEC-1, although with M34 they were much less frequent and did not involve the small bowel. We conclude that AF/R1 pilus expression is not essential for the attaching, effacing lesion but serves as an accessory virulence factor which promotes an initial interaction of RDEC-1 with normal epithelial cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-2442061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-2862689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-2863216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-2863318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-2878887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-2888732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-321703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-3539808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-393628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-4204109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-44705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-4896022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-60092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6103872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6129261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6132950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6136449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6145724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6260374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6274845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6279518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6350186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2899552-6389358
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1846-57
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2899552-Adhesins, Escherichia coli,
pubmed-meshheading:2899552-Animals,
pubmed-meshheading:2899552-Antigens, Bacterial,
pubmed-meshheading:2899552-Bacterial Adhesion,
pubmed-meshheading:2899552-Bacterial Outer Membrane Proteins,
pubmed-meshheading:2899552-Cecum,
pubmed-meshheading:2899552-Chromosome Mapping,
pubmed-meshheading:2899552-Colon,
pubmed-meshheading:2899552-DNA, Bacterial,
pubmed-meshheading:2899552-DNA Mutational Analysis,
pubmed-meshheading:2899552-DNA Transposable Elements,
pubmed-meshheading:2899552-Escherichia coli,
pubmed-meshheading:2899552-Fimbriae, Bacterial,
pubmed-meshheading:2899552-Genes, Bacterial,
pubmed-meshheading:2899552-Ileum,
pubmed-meshheading:2899552-Intestinal Mucosa,
pubmed-meshheading:2899552-Microscopy, Electron,
pubmed-meshheading:2899552-Microvilli,
pubmed-meshheading:2899552-Plasmids,
pubmed-meshheading:2899552-Rabbits
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pubmed:year |
1988
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pubmed:articleTitle |
Characterization of the plasmid from Escherichia coli RDEC-1 that mediates expression of adhesin AF/R1 and evidence that AF/R1 pili promote but are not essential for enteropathogenic disease.
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pubmed:affiliation |
Department of Gastroenterology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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