Linked Life Data

2898966

Source:http://linkedlifedata.com/resource/pubmed/id/2898966

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-8-25
pubmed:abstractText
We report a simple two-step immunocytochemical method that uses staphylococcal protein A conjugated to horseradish peroxidase and the H2O2-3,3'-diaminobenzidine chromogenic reaction to stain neurons and fibers containing immunoreactive cell antigens. Our protein A-peroxidase method was found to produce stains of equal or superior quality to those of the classical 3-step peroxidase-anti-peroxidase (PAP) technique. Since the chromogenic reaction with protein A-peroxidase has low non-specific tissue binding, the reaction can be carried out at acidic pH with higher horseradish peroxidase activity and no significant increase in background. Using this method, we were able to produce clear stains of neurons and fibers containing tyrosine hydroxylase or fibers containing methionine-enkephalin. The use of protein A-peroxidase conjugate substantially simplified the immunocytochemistry procedure, and reduced both the time and cost of experiments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
448
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
192-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
A simple two-step immunocytochemical method using protein A-peroxidase to stain immunoreactive cell antigens.
pubmed:affiliation
Unit Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, MD 20892.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't