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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1988-8-10
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pubmed:abstractText |
The role of GTP on somatostatin-induced K+ current increase was examined in dissociated human pituitary tumor cells obtained from three acromegalic patients. Pituitary cells in culture were voltage-clamped by using the patch clamp technique in the whole-cell configuration. Somatostatin (100 nM) increased the membrane permeability to K+ ions and inhibited hormone secretion. A current-voltage relation of the somatostatin-induced K+ current showed an inward rectification when the concentration of extracellular K+ ions was increased. The amplitude of the somatostatin-induced K+ current decreased during recording when the patch pipette solution did not contain GTP; addition of 100 microM GTP to the patch pipette solution prevented this reduction. Intracellular application of 100 microM guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S] evoked an inward rectifying K+ conductance in the absence of somatostatin. After the GTP[gamma S]-induced K+ conductance reached a steady level, application of somatostatin did not further increase the K+ conductance. In pertussis toxin-treated cells GTP[gamma S] did not evoke K+ conductance. It was concluded that somatostatin-induced K+ channels were regulated by a GTP-binding protein.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2413367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2429251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2431411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2432604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2432660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2433586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2433589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2433590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2440314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2445960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2450994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2456451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2865257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-2874559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-3084531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-6296367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-6296372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-7069629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2898785-7107817
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4924-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2898785-Acromegaly,
pubmed-meshheading:2898785-Adenoma,
pubmed-meshheading:2898785-Drug Interactions,
pubmed-meshheading:2898785-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:2898785-Guanosine Triphosphate,
pubmed-meshheading:2898785-Humans,
pubmed-meshheading:2898785-Membrane Potentials,
pubmed-meshheading:2898785-Pertussis Toxin,
pubmed-meshheading:2898785-Pituitary Neoplasms,
pubmed-meshheading:2898785-Potassium,
pubmed-meshheading:2898785-Somatostatin,
pubmed-meshheading:2898785-Thionucleotides,
pubmed-meshheading:2898785-Tumor Cells, Cultured,
pubmed-meshheading:2898785-Virulence Factors, Bordetella
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pubmed:year |
1988
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pubmed:articleTitle |
Requirement of GTP on somatostatin-induced K+ current in human pituitary tumor cells.
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pubmed:affiliation |
Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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