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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1988-6-9
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pubmed:abstractText |
The place and time of synthesis of the circumsporozoite protein of P. falciparum was analysed with a monoclonal antibody directed against the (NANP)3 repetitive epitope of the CS protein. By using an indirect fluorescent antibody test the epitope could be detected on the oocyst 7 days after infection and 3 days before the appearance of mature sporozoites. Using the Western blot technique, 3 polypeptides from midgut preparations were recognized by the (NANP)3-specific monoclonal antibody from the day 9 of infection onwards. The circumsporozoite precipitation reaction could be induced in sporozoites from either the midgut or the salivary glands 11 days after a blood meal. A similar reactivity of midgut-sporozoites and salivary gland-sporozoites was observed with antisporozoite antisera. Not all sporozoites recovered from the midgut showed a precipitation reaction.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0001-706X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-65
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2896447-Animals,
pubmed-meshheading:2896447-Anopheles,
pubmed-meshheading:2896447-Antibodies, Monoclonal,
pubmed-meshheading:2896447-Chemical Precipitation,
pubmed-meshheading:2896447-Fluorescent Antibody Technique,
pubmed-meshheading:2896447-Humans,
pubmed-meshheading:2896447-Immunoglobulin G,
pubmed-meshheading:2896447-Immunologic Techniques,
pubmed-meshheading:2896447-Mice,
pubmed-meshheading:2896447-Plasmodium falciparum,
pubmed-meshheading:2896447-Protein Biosynthesis,
pubmed-meshheading:2896447-Time Factors
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pubmed:year |
1988
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pubmed:articleTitle |
Formation of the circumsporozoite protein of Plasmodium falciparum in Anopheles stephensi.
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pubmed:affiliation |
Swiss Tropical Institute, Basel, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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