Linked Life Data

2894996

Source:http://linkedlifedata.com/resource/pubmed/id/2894996

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-4-27
pubmed:abstractText
Cell sorting and cytotoxic depletion procedures were used to subdivide the population of CD4- CD8- ("double-negative") thymocytes from adult CBA mice on the basis of expression of Ly-1, HSA (the "heat-stable antigen" M1/69 or B2A2), Pgp-1 glycoprotein, Thy-1, MEL-14 and the PC61 antigenic determinant on the IL2 receptor (IL2R). The level of dividing cells within these subsets was assessed by brief in vivo administration of [3H]-thymidine, followed by radioautography, or by flow cytometric cell cycle analysis after DNA staining. The capacity of the subsets to proliferate in culture, in response to stimulation with concanavalin A (Con A), or with phorbol myristate acetate (PMA) and the calcium ionophore ionomycin, was assessed in high cloning efficiency single-cell culture systems. In general, the proliferative response in culture was inversely related to the rate of cell division in vivo. Response of the double-negative subsets to Con A correlated with expression of the T cell antigen receptor complex; although a high cloning efficiency was obtained from the receptor-positive fractions, very few of the clones were cytotoxic. In particular, a major Ly-1+ HSA- Pgp-1+ double-negative subset, as well as minor Ly-1- HSA- Pgp-1+ subsets, contained very few cells in cycle in vivo, but showed a high cloning efficiency in both culture systems. Conversely, the other major double-negative subset, Ly-1- HSA+ Pgp-1-, included most of the cells in cycle, but showed a reduced cloning efficiency in response to PMA and ionomycin and failed to respond to Con A. The dividing cells within the Ly-1- HSA+ Pgp-1- group were strongly enriched in the IL2R- rather than in the IL2R+ subset, suggesting IL2 was not the growth factor maintaining their proliferation in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2894996-Animals, pubmed-meshheading:2894996-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:2894996-Antigens, Ly, pubmed-meshheading:2894996-Antigens, Surface, pubmed-meshheading:2894996-Antigens, Thy-1, pubmed-meshheading:2894996-Cell Differentiation, pubmed-meshheading:2894996-Cell Division, pubmed-meshheading:2894996-Cell Separation, pubmed-meshheading:2894996-Clone Cells, pubmed-meshheading:2894996-Lymphocyte Activation, pubmed-meshheading:2894996-Male, pubmed-meshheading:2894996-Mice, pubmed-meshheading:2894996-Mice, Inbred CBA, pubmed-meshheading:2894996-Phenotype, pubmed-meshheading:2894996-Receptors, Immunologic, pubmed-meshheading:2894996-Receptors, Interleukin-2, pubmed-meshheading:2894996-Receptors, Lymphocyte Homing, pubmed-meshheading:2894996-T-Lymphocytes, pubmed-meshheading:2894996-T-Lymphocytes, Cytotoxic
pubmed:year
1988
pubmed:articleTitle
Subpopulations of CD4- CD8- murine thymocytes: differences in proliferation rate in vivo and proliferative responses in vitro.
pubmed:affiliation
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't