Linked Life Data

2894393

Source:http://linkedlifedata.com/resource/pubmed/id/2894393

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1988-4-20
pubmed:abstractText
Putative early thymocytes, the Ly-2-L3T4-(CD8-CD4-) cells representing 3 to 4% of adult CBA mouse thymic lymphocytes, were isolated in high purity (99.5%). They were then stained by using mAb and analyzed by flow cytometry for the expression of six additional surface antigenic markers. Cross-correlation of the data obtained from a complete series of successive two-parameter analyses revealed the existence of about 11 discrete subsets, falling into four-main groups, within the Ly-2-L3T4- population. All subsets consisted of relatively large lymphoid cells. The most numerous group of Ly-2-L3T4- cells was Ly-1 low B2A2-M1/69 high Thy-1 high Pgp-1 low and by these markers resembled Ly-2+L3T4+ cortical blasts. Many of the cells in this group were positive for the IL-2R and/or for MEL-14. A second major group of Ly-2-L3T4- cells was Ly-1 high B2A2-M1/69 low Pgp-1 high, and resembled in some respects activated mature T cells. This group had previously been shown to be absent from the embryonic thymus. The group could be divided into Thy-1 high and Thy-1 low subsets. None of the cells in this group were positive for the IL-2R and very few expressed MEL-14. A third group, 13% of the Ly-2-L3T4- population, was Ly-1 low B2A2-M1/69 low Pgp-1 high, and could also be divided into Thy-1 high and Thy-1 low subsets. A final minor group, 9% of the Ly-2-L3T4- population, was Ly-1 high B2A2-M1/69 high Pgp-1 low Thy-1 high. The particular pattern of markers on these subsets, combined with subsequent information on their properties, makes it unlikely that they all represent sequential steps in one continuous developmental stream, and indicates that complex developmental steps have occurred, even at this supposedly early stage of T cell differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
140
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1461-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2894393-Aging, pubmed-meshheading:2894393-Animals, pubmed-meshheading:2894393-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:2894393-Antigens, Ly, pubmed-meshheading:2894393-Antigens, Surface, pubmed-meshheading:2894393-Antigens, Thy-1, pubmed-meshheading:2894393-Cell Adhesion Molecules, pubmed-meshheading:2894393-Cell Differentiation, pubmed-meshheading:2894393-Cell Separation, pubmed-meshheading:2894393-Flow Cytometry, pubmed-meshheading:2894393-Male, pubmed-meshheading:2894393-Membrane Glycoproteins, pubmed-meshheading:2894393-Mice, pubmed-meshheading:2894393-Mice, Inbred CBA, pubmed-meshheading:2894393-Phenotype, pubmed-meshheading:2894393-Receptors, Immunologic, pubmed-meshheading:2894393-Receptors, Interleukin-2, pubmed-meshheading:2894393-T-Lymphocytes, pubmed-meshheading:2894393-Thymus Gland
pubmed:year
1988
pubmed:articleTitle
Subpopulations of early thymocytes. A cross-correlation flow cytometric analysis of adult mouse Ly-2-L3T4-(CD8-CD4-) thymocytes using eight different surface markers.
pubmed:affiliation
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't