Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1987-11-25
|
| pubmed:abstractText |
Restriction fragment length polymorphism (RFLP) of Ha-ras gene was surveyed by Southern blot analysis in leukocyte DNA from 55 normal individuals and 58 urothelial cancer patients in Japan. Three common alleles and 4 rare alleles were classified. The frequency of common alleles in normal Japanese individuals differed from that in Caucasians previously reported; a 7.2-7.5-kb BamHI fragment of common allele was not observed in Japanese individuals. No significant increase in frequency of the rare Ha-ras allele was observed in the group of cancer patients. Moreover, no significant difference in frequency was observed for the 3 common alleles. Tumor DNA was compared with leukocyte DNA in 30 urothelial cancer patients: in 3 of 8 cases with heterozygous Ha-ras locus, decreased intensity of one band, indicating partial loss of one allele in tumor DNA, was observed. In 3 tumors with either deletion of one Ha-ras allele or a rare Ha-ras allele, expression of Ha-ras gene was examined by Northern blot analysis. Such genetic alterations did not always result in a marked increase in Ha-ras expression. These data suggest that these genetic alterations are not directly related to Ha-ras expression, and that RFLP of Ha-ras gene is not a useful genetic marker for urothelial cancer. On the other hand, deletion of one Ha-ras allele was observed in 1 of 5 cases of bladder cancer and in 2 of 3 cases of renal pelvic cancer, suggesting that that deletion may be important in the development of urothelial cancer.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease BamHI,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
40
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
474-8
|
| pubmed:dateRevised |
2007-7-24
|
| pubmed:meshHeading |
pubmed-meshheading:2889677-Aged,
pubmed-meshheading:2889677-Alleles,
pubmed-meshheading:2889677-Cell Line,
pubmed-meshheading:2889677-DNA,
pubmed-meshheading:2889677-DNA, Neoplasm,
pubmed-meshheading:2889677-DNA Restriction Enzymes,
pubmed-meshheading:2889677-Deoxyribonuclease BamHI,
pubmed-meshheading:2889677-European Continental Ancestry Group,
pubmed-meshheading:2889677-Female,
pubmed-meshheading:2889677-Gene Expression Regulation,
pubmed-meshheading:2889677-Humans,
pubmed-meshheading:2889677-Leukocytes,
pubmed-meshheading:2889677-Male,
pubmed-meshheading:2889677-Middle Aged,
pubmed-meshheading:2889677-Oceanic Ancestry Group,
pubmed-meshheading:2889677-Oncogenes,
pubmed-meshheading:2889677-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:2889677-RNA, Neoplasm,
pubmed-meshheading:2889677-Urinary Bladder Neoplasms
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Lack of correlation between rare Ha-ras alleles and urothelial cancer in Japan.
|
| pubmed:affiliation |
Department of Pathology, Kobe University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|