Linked Life Data

2887480

Source:http://linkedlifedata.com/resource/pubmed/id/2887480

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1987-10-9
pubmed:abstractText
Enteroglucagon-containing cells have been maintained in short-term culture, and the morphologic characteristics of these cells and their response to selected agents have been determined. After 48 h in culture the ultrastructural appearance of the enteroglucagon-immunoreactive cells showed evidence of polarization with re-formation of apical microvilli and the secretory granules concentrated at the opposite pole of the cell. The size of the intracellular secretory granules was 370 +/- 15 nm. The release of enteroglucagonlike immunoreactivity was stimulated in a dose-dependent manner by the adrenergic agonists epinephrine and isoproterenol. The response to epinephrine was competitively inhibited by propranolol, producing a rightward shift of the dose-responsive curve. The alpha-adrenergic agonists methoxamine and clonidine did not stimulate enteroglucagon release above basal. The adenyl cyclase activator forskolin also stimulated release of the peptide in a dose-dependent manner. Carbachol and somatostatin produced a dose-dependent inhibition of epinephrine-stimulated release, indicating direct inhibitory modulation of enteroglucagonlike immunoreactive cells. Somatostatin also inhibited forskolin-stimulated release. These data indicate that canine ileal enteroglucagon cells in short-term culture respond to a number of specific stimuli.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0016-5085
pubmed:author
pubmed:issnType
Print
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
791-800
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Morphologic and physiologic studies of canine ileal enteroglucagon-containing cells in short-term culture.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't