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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1987-6-8
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pubmed:abstractText |
This study reports on the synthesis of two fluorescent analogues of thymopentin (TP-5; Arg-Lys-Asp-Val-Tyr). A fluorescein isothiocyanate labeled analogue (FITC-TP-5) and a stilbene isothiocyanate labeled analogue (SITS-TP-5) were extensively purified by ion-exchange and gel filtration chromatography. Characterization of the coupling site through amino acid analysis, dansylation and N-terminal cleavage of the fluorescent amino acid yielded results which indicated that both were mono-labeled analogues derivatized at the N-terminal. These analogues were shown to be TP-5-like in nature by their ability to induce the expression of the Thy 1.2 surface marker on nude mouse prothymocytes in both in vivo and in vitro assays. In addition, these analogues were able to inhibit the specific binding of radiolabeled TP-5 to human lymphocytes. Initial studies describing the interaction of FITC-TP-5 with human lymphocytes are shown.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-Acetamido-4'-isothiocyanatostilben...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Stilbenes,
http://linkedlifedata.com/resource/pubmed/chemical/Thymopentin,
http://linkedlifedata.com/resource/pubmed/chemical/Thymopoietins,
http://linkedlifedata.com/resource/pubmed/chemical/Thymus Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/thymopoietin receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0367-8377
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-86
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2883150-4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid,
pubmed-meshheading:2883150-Animals,
pubmed-meshheading:2883150-Antigens, Surface,
pubmed-meshheading:2883150-Antigens, Thy-1,
pubmed-meshheading:2883150-Fluorescein-5-isothiocyanate,
pubmed-meshheading:2883150-Fluoresceins,
pubmed-meshheading:2883150-Indicators and Reagents,
pubmed-meshheading:2883150-Mice,
pubmed-meshheading:2883150-Mice, Nude,
pubmed-meshheading:2883150-Peptide Fragments,
pubmed-meshheading:2883150-Receptors, Cell Surface,
pubmed-meshheading:2883150-Receptors, Peptide,
pubmed-meshheading:2883150-Stilbenes,
pubmed-meshheading:2883150-Structure-Activity Relationship,
pubmed-meshheading:2883150-T-Lymphocytes,
pubmed-meshheading:2883150-Thymopentin,
pubmed-meshheading:2883150-Thymopoietins,
pubmed-meshheading:2883150-Thymus Hormones
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pubmed:year |
1987
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pubmed:articleTitle |
Synthesis of two biologically active fluorescent probes of thymopentin.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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