Linked Life Data

2878934

Source:http://linkedlifedata.com/resource/pubmed/id/2878934

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-2-19
pubmed:abstractText
It is well known that in patients with Graves' disease there is dissociation among values for TSH receptor antibodies such as TSH binding-inhibiting antibody (TBIAb) and thyroid-stimulating antibody (TSAb). This study was designed to compare TSH receptor antibodies in serum and in culture supernatants of lymphocytes transformed by Epstein-Barr virus (EBV) infection. TBIAb was assayed using solubilized porcine thyroid membranes and TSAb using cultured porcine thyroid cells. Transformation of peripheral lymphocytes by EBV was performed in eight untreated hyperthyroid patients with Graves' disease who had a clear dissociation between values for TBIAb and TSAb, even after multiple dilutions of serum. EBV-infected lymphocytes from a patient were distributed into the wells of 4 24-well culture plates. After the first 4-week culture, the cells in each well positive for TBIAb and/or TSAb were further distributed to 12 wells and cultured for 2 weeks. In the supernatants of 372 wells of the second culture in the 8 patients, 31 were positive for TBIAb, and 41 were positive for TSAb. Among the 76 wells positive for TSH receptor antibodies, 72 wells (94.7%) were positive in only 1 assay. These results suggest that there are two types of TSH receptor antibodies, one having a single function (TBIAb or TSAb) and the other multifunctional (both), in Graves' patients. The incidence of the single functional type is much greater than that of the multifunctional type. We conclude, therefore, that the heterogeneity of Graves' immunoglobulin G is due mainly to multiple antibodies with a single function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-972X
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Heterogeneity of Graves' immunoglobulin G: comparison of thyrotropin receptor antibodies in serum and in culture supernatants of lymphocytes transformed by Epstein-Barr virus infection.
pubmed:publicationType
Journal Article, Comparative Study