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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-12-30
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pubmed:abstractText |
Recently we have described the molecular cloning of the genetic determinant coding for the S-fimbrial adhesin (Sfa), a sialic acid-recognizing pilus frequently found among extraintestinal Escherichia coli isolates. Fimbriae from the resulting Sfa+ E. coli K-12 clone were isolated, and an Sfa-specific antiserum was prepared. Western blots indicate that S fimbriae isolated from different uropathogenic and meningitis-associated E. coli strains, including O83:K1 isolates, were serologically related. The Sfa-specific antibodies did not cross-react with P fimbriae, but did cross-react with F1C fimbriae. Furthermore the sfa+ recombinant DNAs and some cloned sfa-flanking regions were used as probes in Southern experiments. Chromosomal DNAs isolated from O18:K1 and O83:K1 meningitis strains with and without S fimbriae and from uropathogenic O6:K+ strains were hybridized against these sfa-specific probes. Only one copy of the sfa determinant was identified on the chromosome of these strains. No sfa-specific sequences were observed on the chromosome of E. coli K-12 strains and an O7:K1 isolate. With the exception of small alterations in the sfa-coding region the genetic determinants for S fimbriae were identical in uropathogenic O6:K+ and meningitis O18:K1 and O83:K1 strains. The sfa determinant was also detected on the chromosome of K1 isolates with an Sfa-negative phenotype, and specific cross-hybridization signals were visible after blotting against F1C-specific DNA. In addition homology among the different strains was observed in the sfa-flanking regions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2877948-105244,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
646-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2877948-Adhesins, Escherichia coli,
pubmed-meshheading:2877948-Antigens, Bacterial,
pubmed-meshheading:2877948-Bacterial Adhesion,
pubmed-meshheading:2877948-Bacterial Proteins,
pubmed-meshheading:2877948-Chromosome Mapping,
pubmed-meshheading:2877948-DNA, Bacterial,
pubmed-meshheading:2877948-Escherichia coli,
pubmed-meshheading:2877948-Fimbriae, Bacterial,
pubmed-meshheading:2877948-Genes, Bacterial,
pubmed-meshheading:2877948-Humans,
pubmed-meshheading:2877948-Meningitis,
pubmed-meshheading:2877948-Molecular Weight,
pubmed-meshheading:2877948-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2877948-Sialic Acids,
pubmed-meshheading:2877948-Species Specificity,
pubmed-meshheading:2877948-Urinary Tract Infections
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pubmed:year |
1986
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pubmed:articleTitle |
Analysis of the genetic determinants coding for the S-fimbrial adhesin (sfa) in different Escherichia coli strains causing meningitis or urinary tract infections.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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