Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-7-17
pubmed:abstractText
Cultures of central-nervous-system neurons at low densities require for their survival exogenous pyruvate, alpha-oxoglutarate or oxaloacetate, even in the presence of high glucose concentrations. Most other alpha-oxo acids support cell survival only in the presence of alpha-amino acids which transaminate to alpha-oxoglutarate, oxaloacetate or pyruvate. The alpha-oxo acids therefore operate as acceptors of amino groups from appropriate donors to generate tricarboxylic acid-cycle-relevant substrates, and these alpha-oxo acids provide for neuronal support only insofar as they make it possible for exogenously supplied alpha-amino acid precursors to generate intracellularly one of the three critical metabolites. To examine more closely the relationship between transamination activity and neuronal survival, we measured 14CO2 production from [14C]glutamate in the presence of appropriate alpha-oxo acid partners by using 8-day-embryonic chick forebrain, dorsal-root-ganglion and ciliary-ganglion neurons. Neuronal survival was measured concurrently in monolayer neuronal cultures maintained with the corresponding amino acid/oxo acid pairs. Forebrain and ganglionic cell suspensions both produced 14CO2 from [14C]glutamate, which accurately correlated with 24 h neuronal survival. Concentrations of glutamate or alpha-oxo acid which provide for maximal neuronal survival also produced maximal amounts of 14CO2. The same ability to generate CO2 from glutamate (in the presence of the appropriate alpha-oxo acids) can ensure neuronal survival in 24 h cultures and therefore must meet energy or other metabolic needs of those neurons which glucose itself is unable to satisfy.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-13554627, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-3917493, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-4031868, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-535534, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-575536, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6282902, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6311327, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6368763, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6428706, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6491662, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6860949, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-7276956, http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-985377
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
234
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
605-10
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Transamination of glutamate to tricarboxylic acid-cycle intermediates in cultured neurons correlates with the ability of oxo acids to support neuronal survival in vitro.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't