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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001128,
umls-concept:C0010453,
umls-concept:C0027882,
umls-concept:C0038952,
umls-concept:C0085732,
umls-concept:C0183683,
umls-concept:C0205103,
umls-concept:C0220839,
umls-concept:C0344211,
umls-concept:C0521390,
umls-concept:C0597599,
umls-concept:C1171411,
umls-concept:C1317973,
umls-concept:C1521721,
umls-concept:C1533691
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pubmed:issue |
3
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pubmed:dateCreated |
1986-7-17
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pubmed:abstractText |
Cultures of central-nervous-system neurons at low densities require for their survival exogenous pyruvate, alpha-oxoglutarate or oxaloacetate, even in the presence of high glucose concentrations. Most other alpha-oxo acids support cell survival only in the presence of alpha-amino acids which transaminate to alpha-oxoglutarate, oxaloacetate or pyruvate. The alpha-oxo acids therefore operate as acceptors of amino groups from appropriate donors to generate tricarboxylic acid-cycle-relevant substrates, and these alpha-oxo acids provide for neuronal support only insofar as they make it possible for exogenously supplied alpha-amino acid precursors to generate intracellularly one of the three critical metabolites. To examine more closely the relationship between transamination activity and neuronal survival, we measured 14CO2 production from [14C]glutamate in the presence of appropriate alpha-oxo acid partners by using 8-day-embryonic chick forebrain, dorsal-root-ganglion and ciliary-ganglion neurons. Neuronal survival was measured concurrently in monolayer neuronal cultures maintained with the corresponding amino acid/oxo acid pairs. Forebrain and ganglionic cell suspensions both produced 14CO2 from [14C]glutamate, which accurately correlated with 24 h neuronal survival. Concentrations of glutamate or alpha-oxo acid which provide for maximal neuronal survival also produced maximal amounts of 14CO2. The same ability to generate CO2 from glutamate (in the presence of the appropriate alpha-oxo acids) can ensure neuronal survival in 24 h cultures and therefore must meet energy or other metabolic needs of those neurons which glucose itself is unable to satisfy.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-13554627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-3917493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-4031868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-535534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-575536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6282902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6311327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6368763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6428706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6491662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-6860949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-7276956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2872884-985377
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0264-6021
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
234
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
605-10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2872884-Animals,
pubmed-meshheading:2872884-Brain,
pubmed-meshheading:2872884-Carbon Dioxide,
pubmed-meshheading:2872884-Cell Survival,
pubmed-meshheading:2872884-Cells, Cultured,
pubmed-meshheading:2872884-Chick Embryo,
pubmed-meshheading:2872884-Citric Acid Cycle,
pubmed-meshheading:2872884-Glutamates,
pubmed-meshheading:2872884-Glutamic Acid,
pubmed-meshheading:2872884-Keto Acids,
pubmed-meshheading:2872884-Neurons
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pubmed:year |
1986
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pubmed:articleTitle |
Transamination of glutamate to tricarboxylic acid-cycle intermediates in cultured neurons correlates with the ability of oxo acids to support neuronal survival in vitro.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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