Linked Life Data

2871822

Source:http://linkedlifedata.com/resource/pubmed/id/2871822

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1986-5-28
pubmed:abstractText
Immunoadsorbents were obtained by coupling antibodies to Sepharose 4 B activated with cyanogen bromide. Thus immobilized antibody directed against bovine kidney gamma-glutamyltransferase was used for immunoaffinity chromatography of the enzyme from bovine kidney and liver, from cow milk and from sheep kidney and liver. Immobilized anti-rat kidney gamma-glutamyltransferase antibody was used for purification of the enzyme from rat kidney, mouse kidney, hamster kidney and rat Morris hepatoma 5123D. Yields of the protease-solubilized gamma-glutamyltransferases isolated on immunoadsorbents columns were usually over 60%. The purified enzymes were almost homogenous in polyacrylamide gel electrophoresis. The enzymes showed different molecular weights and electrophoretic mobilities. The effect of antibodies on affinity of the enzymes to substrate and inhibition by synthetic anthglutin and its isomers were studied.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0004-069X
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
715-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Purification of gamma-glutamyltransferases by immunoaffinity chromatography and some properties of the enzymes.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't