Linked Life Data

2871761

Source:http://linkedlifedata.com/resource/pubmed/id/2871761

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
1986-6-18
pubmed:abstractText
The present study compared the effects of insulin, 2-chloroadenosine, and prostaglandin E2 as inhibitors of respiration in hamster brown adipocytes stimulated with either isoproterenol or phenylephrine. Addition of 2-chloroadenosine or prostaglandin E2 strongly antagonized isoproterenol-stimulated respiration; phenylephrine-stimulated respiration was also partially inhibited by 2-chloroadenosine and prostaglandin E2, but the extent of inhibition caused by these agents was not as great as when isoproterenol was used. Isoproterenol-stimulated respiration was inhibited by insulin, but phenylephrine-stimulated respiration was insensitive to the inhibitory effect of insulin. When brown adipocytes were pretreated with pertussis toxin, isoproterenol-stimulated respiration was enhanced, but phenylephrine-stimulated respiration was not significantly affected. The inhibitory effects of 2-chloroadenosine and prostaglandin E2 on isoproterenol-stimulated respiration were completely blocked by pertussis toxin, indicating that the mode of action of these inhibitory hormones was secondary to inhibition of adenylate cyclase and resultant inhibition of lipolysis. Prostaglandin E2 inhibition of phenylephrine-stimulated respiration was also abolished by pertussis toxin. In contrast, 2-chloroadenosine inhibition of phenylephrine-stimulated respiration persisted in adipocytes treated with pertussis toxin. These data suggest that phenylephrine stimulates respiration through a mechanism that is not altered by pertussis toxin and further that 2-chloroadenosine inhibition of isoproterenol- or phenylephrine-stimulated respiration can be dissociated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/2-Chloroadenosine, http://linkedlifedata.com/resource/pubmed/chemical/Adenosine, http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase, http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic alpha-Agonists, http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone, http://linkedlifedata.com/resource/pubmed/chemical/Insulin, http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol, http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Phenylephrine, http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandins E, http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
250
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C738-43
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Effects of insulin, adenosine, and prostaglandin on alpha-adrenergic-stimulated respiration in brown adipocytes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.