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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1986-6-6
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pubmed:abstractText |
This study examines the potential role of transforming growth factor beta (TGF-beta) in the regulation of human T lymphocyte proliferation, and proposes that TGF-beta is an important autoregulatory lymphokine that limits T lymphocyte clonal expansion, and that TGF-beta production by T lymphocytes is important in T cell interactions with other cell types. TGF-beta was shown to inhibit IL-2-dependent T cell proliferation. The addition of picograms amounts of TGF-beta to cultures of IL-2-stimulated human T lymphocytes suppressed DNA synthesis by 60-80%. A potential mechanism of this inhibition was found. TGF-beta inhibited IL-2-induced upregulation of the IL-2 and transferrin receptors. Specific high-affinity receptors for TGF-beta were found both on resting and activated T cells. Cellular activation was shown to result in a five- to sixfold increase in the number of TGF-beta receptors on a per cell basis, without a change in the affinity of the receptor. Finally, the observations that activated T cells produce TGF-beta mRNA and that TGF-beta biologic activity is present in supernatants conditioned by activated T cells is strong evidence that T cells themselves are a source of TGF-beta. Resting T cells were found to have low to undetectable levels of TGF-beta mRNA, while PHA activation resulted in a rapid increase in TGF-beta mRNA levels (within 2 h). Both T4 and T8 lymphocytes were found to make mRNA for TGF-beta upon activation. Using both a soft agar assay and a competitive binding assay, TGF-beta biologic activity was found in supernatants conditioned by T cells; T cell activation resulted in a 10-50-fold increase in TGF-beta production. Thus, TGF-beta may be an important antigen-nonspecific regulator of human T cell proliferation, and important in T cell interaction with other cell types whose cellular functions are modulated by TGF-beta.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-1078737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-273914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-2983318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-2985512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-2987968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-3871521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-4229841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-450142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-4542806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-549778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6088525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6092510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6093254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6096259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6208555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6211485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6231642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6270680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6280171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6303865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6319010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6367046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6429853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6451643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6572416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6602130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6602340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6604914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6606011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6607069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6609200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6957865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6975347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-6975480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2871125-7000676
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transferrin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transforming Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factors
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1037-50
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2871125-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2871125-Antigens, Surface,
pubmed-meshheading:2871125-Cell Cycle,
pubmed-meshheading:2871125-Dose-Response Relationship, Drug,
pubmed-meshheading:2871125-Gene Expression Regulation,
pubmed-meshheading:2871125-Humans,
pubmed-meshheading:2871125-Interleukin-2,
pubmed-meshheading:2871125-Kinetics,
pubmed-meshheading:2871125-Lymphocyte Activation,
pubmed-meshheading:2871125-Peptide Biosynthesis,
pubmed-meshheading:2871125-Peptides,
pubmed-meshheading:2871125-RNA, Messenger,
pubmed-meshheading:2871125-Receptors, Cell Surface,
pubmed-meshheading:2871125-Receptors, Immunologic,
pubmed-meshheading:2871125-Receptors, Interleukin-2,
pubmed-meshheading:2871125-Receptors, Transferrin,
pubmed-meshheading:2871125-Receptors, Transforming Growth Factor beta,
pubmed-meshheading:2871125-T-Lymphocytes,
pubmed-meshheading:2871125-Transforming Growth Factors
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pubmed:year |
1986
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pubmed:articleTitle |
Production of transforming growth factor beta by human T lymphocytes and its potential role in the regulation of T cell growth.
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