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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1986-5-8
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pubmed:abstractText |
A series of hybrids between primary human cells and a Chinese hamster somatic cell mutant (Mev-1), defective in expression of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase [(S)-3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acetylating, EC 4.1.3.5], has been prepared that complements the mutant defect. A technique based on differential sensitivity of this enzyme activity to inhibition by magnesium ion is described that allows the discrimination of expression of human and hamster HMG-CoA synthase in these hybrids. The results indicate a structural gene defect in expression of HMG-CoA synthase activity in Mev-1 cells. Segregation of human chromosomes that do not possess the complementing marker have allowed the assignment of human HMG-CoA synthase activity to chromosome 5. This is the second demonstrably transcriptionally regulated enzyme of cholesterologenesis to be assigned to chromosome 5, the other being HMG-CoA reductase.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-228272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-2995526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-4132053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-438201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-4524661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-6105157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-6139093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-6397553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-6501306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-6565705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-6863296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-6934068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-6961444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-7023367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-7085625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-7197058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-7391033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2870496-818332
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2187-9
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pubmed:dateRevised |
2011-11-4
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pubmed:meshHeading |
pubmed-meshheading:2870496-Chromosome Mapping,
pubmed-meshheading:2870496-Chromosomes, Human, 4-5,
pubmed-meshheading:2870496-Gene Expression Regulation,
pubmed-meshheading:2870496-Humans,
pubmed-meshheading:2870496-Hybrid Cells,
pubmed-meshheading:2870496-Hydroxymethylglutaryl-CoA Synthase,
pubmed-meshheading:2870496-Magnesium,
pubmed-meshheading:2870496-Oxo-Acid-Lyases,
pubmed-meshheading:2870496-Sterols
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pubmed:year |
1986
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pubmed:articleTitle |
Localization of the gene encoding 3-hydroxy-3-methylglutaryl-coenzyme A synthase to human chromosome 5.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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