rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-3-14
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pubmed:abstractText |
Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion for 3 min with galactosylated cholinesterase, a ligand for the asialoglycoprotein receptor. Highly enriched populations of endocytic and exocytic coated vesicles can be separated by use of a novel cholinesterase mediated density shift technique. The two coated vesicle classes have very similar polypeptide compositions but differ significantly in the ratio of cholesterol to phospholipid.
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pubmed:grant |
|
pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0092-8674
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
14
|
pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
497-506
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2867830-Acetylcholinesterase,
pubmed-meshheading:2867830-Animals,
pubmed-meshheading:2867830-Butyrylcholinesterase,
pubmed-meshheading:2867830-Cell Fractionation,
pubmed-meshheading:2867830-Centrifugation, Density Gradient,
pubmed-meshheading:2867830-Cholesterol,
pubmed-meshheading:2867830-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2867830-Endocytosis,
pubmed-meshheading:2867830-Endosomes,
pubmed-meshheading:2867830-Exocytosis,
pubmed-meshheading:2867830-Galactose,
pubmed-meshheading:2867830-Isoflurophate,
pubmed-meshheading:2867830-Liver,
pubmed-meshheading:2867830-Phospholipids,
pubmed-meshheading:2867830-Rats,
pubmed-meshheading:2867830-Rats, Inbred Strains
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pubmed:year |
1986
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pubmed:articleTitle |
Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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