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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1985-11-18
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pubmed:abstractText |
The molecular forms of somatostatin contained in the rat striatum were separated by size-exclusion HPLC. Three major peaks of somatostatin-like immunoreactivity (SLI) were resolved. Two peaks cochromatographed with synthetic somatostatin-14 (SS-14) and somatostatin-28 (SS-28), respectively. One peak exhibited a higher molecular weight (about 10,000) and may contain a proform of somatostatin. Local injection of the neurotoxin kainic acid (1 microgram) into the left striatum resulted in a persistent decrease (65-85%) of all three forms of somatostatin. In the contralateral--not injected--striatum a decrease of SLI was also observed which was maximal (45%) after 2 days and was largely abolished after 7 days. This decrease of SLI in the contralateral striatum, however, was due mainly to a decrease of SS-14 and SS-28 but not of the putative proform. Our data suggest that kainic acid causes a destruction of somatostatin-containing perikarya in the injected striatum, whereas in the contralateral striatum increased release with subsequent inactivation of SS-14 and SS-28 takes place. The putative somatostatin proform may serve as neurochemical marker for somatostatin-containing perikarya in the striatum.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-3042
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1441-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2864394-Animals,
pubmed-meshheading:2864394-Chromatography, High Pressure Liquid,
pubmed-meshheading:2864394-Corpus Striatum,
pubmed-meshheading:2864394-Dose-Response Relationship, Drug,
pubmed-meshheading:2864394-Kainic Acid,
pubmed-meshheading:2864394-Kinetics,
pubmed-meshheading:2864394-Male,
pubmed-meshheading:2864394-Protein Precursors,
pubmed-meshheading:2864394-Radioimmunoassay,
pubmed-meshheading:2864394-Rats,
pubmed-meshheading:2864394-Rats, Inbred Strains,
pubmed-meshheading:2864394-Somatostatin,
pubmed-meshheading:2864394-Somatostatin-28
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pubmed:year |
1985
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pubmed:articleTitle |
Somatostatin precursor in the rat striatum: changes after local injection of kainic acid.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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