Linked Life Data

2864349

Source:http://linkedlifedata.com/resource/pubmed/id/2864349

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-11-21
pubmed:abstractText
Previously we reported that cultured human fibroblasts depleted of intracellular potassium (K+) had a reduced number of surface coated pits and were unable to internalize receptor-bound molecules such as low density lipoprotein (LDL). We have extended these studies in two important ways. First, we have developed a method for modulating the number of coated pits in situ. Human fibroblasts incubated in K+-free buffer that contains 4 micron nigericin rapidly become depleted of K+ and lose the ability to internalize 125I-LDL. When rat livers are perfused with the same buffer, there is a 75% decrease in the number of surface coated pits in hepatocytes. Secondly, we have explored the possibility that K+-depletion effects coated pit function by lowering intracellular ATP. We found that although this protocol lowers intracellular ATP by 40-70%, when ATP concentrations are lowered greater than 95% by metabolic inhibitors, receptor-mediated endocytosis is unaffected.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
372-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Modulation of intracellular potassium and ATP: effects on coated pit function in fibroblasts and hepatocytes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't