Linked Life Data

2857621

Source:http://linkedlifedata.com/resource/pubmed/id/2857621

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1985-4-1
pubmed:abstractText
The effects of varying [Ca2+]o on the contraction of smooth muscle by different alpha-adrenoceptor agonists were examined on rat isolated anococcygeus muscle. Agonists were tested in the presence of various [Ca2+]o or 'Ca2+-re-addition curves' were constructed. In some experiments the [Ca2+]free was buffered with EGTA and nitrilotriacetic acid. The components of the response which were revealed were further analysed by using drugs which modify Ca2+ mobilization. Three separate elements in the contractile response were identified: (i) an initial transient contraction, due to intracellular Ca2+ release could be isolated with [Ca2+]o between 1 nmol/l and 3 mumol/l (this could be obtained only with noradrenaline, phenylephrine and amidephrine); (ii) a nifedipine-sensitive response requiring [Ca2+]o of 3 mumol/l or more; (iii) a nifedipine-resistant response requiring [Ca2+]o of 100 mumol/l or more. Presumably (ii) and (iii) involve the entry of Ca2+o: they could be obtained with all agonists tested, including these above, methoxamine, indanidine and xylazine. The results are discussed in relation to the possibility of distinct types of response and their relationship to subgroups of receptors or agonists. It is concluded that there is a continuous spectrum of activity across the agonist range and that this is likely to correlate with 'efficacy' at a single alpha 1 receptor type.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0143-5221
pubmed:author
pubmed:issnType
Print
pubmed:volume
68 Suppl 10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55s-63s
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
alpha-Adrenoceptor agonists and the Ca2+-dependence of smooth muscle contraction: evidence for subtypes of receptors or for agonist-dependent differences in the agonist-receptor interaction?
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't