Linked Life Data

2851601

Source:http://linkedlifedata.com/resource/pubmed/id/2851601

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1989-3-23
pubmed:abstractText
In a study of the infection of mouse embryo fibroblasts with murine cytomegalovirus (MCMV), we found that plaque number is directly related to virus concentration and not to the total amount of virus contained in the inoculum. These results suggested that virus binding was reversible and that during infection a binding equilibrium is established which limits the amount of bound virus. Further analysis revealed three categories of plaque based on reversibility after virus adsorption. One group was removed simply by washing cell monolayers after virus removal. A second group of plaques was lost gradually with time, giving complete reversal after 5 min at 37 degrees C. The rate of reversal was temperature dependent, and probably represented true virus dissociation. The final group was irreversible plaques, the number of which increased with increasing infection time. The number of reversible plaques remained constant with time of infection, and represented about 70% of the total plaques after 1 h of virus adsorption. Centrifugation of the virus inoculum onto the fibroblast monolayer at 1000 X g increased plaque numbers up to 100-fold, but had little effect on plaque number when carried out after the virus inoculum was removed. In contrast centrifugation increased the number of reversible plaques, suggesting an increase in the number of virus particles attached to the cell monolayers. We suggest that centrifugation enhances MCMV infection by three mechanisms related to reversibility of binding: (1), it increases the rate of virus association; (2), it decreases the rate of dissociation; (3), by increasing the length of time each virus particle is bound it increases the probability of virus being taken into the cell.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
215-30
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Murine cytomegalovirus binds reversibly to mouse embryo fibroblasts: implications for quantitation and explanation of centrifugal enhancement.
pubmed:affiliation
Department of Microbiology, University of Western Australia, Nedlands, Perth.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't