| pubmed-article:2851540 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2851540 | lifeskim:mentions | umls-concept:C0024109 | lld:lifeskim |
| pubmed-article:2851540 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2851540 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:2851540 | lifeskim:mentions | umls-concept:C0025519 | lld:lifeskim |
| pubmed-article:2851540 | lifeskim:mentions | umls-concept:C0023545 | lld:lifeskim |
| pubmed-article:2851540 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:2851540 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:2851540 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:2851540 | pubmed:dateCreated | 1989-3-16 | lld:pubmed |
| pubmed-article:2851540 | pubmed:abstractText | We studied the generation and metabolism of leukotrienes (LTs) in human lung macrophages obtained from lung tissue of patients with central bronchial carcinoma. By counterflow centrifugation macrophages were enriched with a purity of more than 95-100%. A time and dose dependent generation of LTB4 and LTC4 was determined by specific radioimmunoassays after stimulation with the Ca-ionophore and anti-IgE. The amount of LTB4 exceeded the amount of LTC4. The concentrations of leukotrienes in the macrophage fraction amounted to 4.3 +/- 2.2 ng LTB4 and 0.6 +/- 0.05 ng LTC4/1 x 10(7) cells after 5 min of incubation with the Ca-ionophore. The LTB4 levels decreased to 3.0 +/- 0.6 ng after 60 min indicating the metabolism of the generated LTB4 by human lung macrophages. This was confirmed by incubation of the cells with exogenously added [3H]LTB4. LTB4 was converted into unpolar products as was identified by thin-layer chromatography and high-performance liquid chromatography; a comparison with the fibroblast cell line L929 which is known to convert LTB4 into the dihydro-LTB4 metabolite (5,12-dihydroxyeicosatrienoic acid) indicates that human lung macrophages use the same pathway of metabolization. Biological inactivation as determined by chemotaxis and cross-reaction with the LTB4 antiserum correlates with the degree of LTB4 metabolism. Moreover, the macrophages convert LTC4 into LTD4 and LTE4 by the enzymatic activity of the gamma-glutamyltranspeptidase and dipeptidase. Our data emphasize that the human alveolar macrophage not only produces arachidonic acid metabolites but modulates the local inflammatory potential by its metabolizing capacity for leukotrienes. | lld:pubmed |
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| pubmed-article:2851540 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2851540 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2851540 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2851540 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2851540 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2851540 | pubmed:issn | 0019-2805 | lld:pubmed |
| pubmed-article:2851540 | pubmed:author | pubmed-author:KönigWW | lld:pubmed |
| pubmed-article:2851540 | pubmed:author | pubmed-author:HilgerRR | lld:pubmed |
| pubmed-article:2851540 | pubmed:author | pubmed-author:SchlüterBB | lld:pubmed |
| pubmed-article:2851540 | pubmed:author | pubmed-author:SchönfeldWW | lld:pubmed |
| pubmed-article:2851540 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2851540 | pubmed:volume | 65 | lld:pubmed |
| pubmed-article:2851540 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2851540 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2851540 | pubmed:pagination | 529-36 | lld:pubmed |
| pubmed-article:2851540 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2851540 | pubmed:meshHeading | pubmed-meshheading:2851540-... | lld:pubmed |
| pubmed-article:2851540 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2851540 | pubmed:articleTitle | Leukotriene generation and metabolism in isolated human lung macrophages. | lld:pubmed |
| pubmed-article:2851540 | pubmed:affiliation | Lehrstuhl für Medizinische Mikrobiologie und Immunologie, AG Infektabwehrmechanismen, Ruhr Universität Bochum, FRG. | lld:pubmed |
| pubmed-article:2851540 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2851540 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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