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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1989-3-16
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pubmed:abstractText |
We studied the generation and metabolism of leukotrienes (LTs) in human lung macrophages obtained from lung tissue of patients with central bronchial carcinoma. By counterflow centrifugation macrophages were enriched with a purity of more than 95-100%. A time and dose dependent generation of LTB4 and LTC4 was determined by specific radioimmunoassays after stimulation with the Ca-ionophore and anti-IgE. The amount of LTB4 exceeded the amount of LTC4. The concentrations of leukotrienes in the macrophage fraction amounted to 4.3 +/- 2.2 ng LTB4 and 0.6 +/- 0.05 ng LTC4/1 x 10(7) cells after 5 min of incubation with the Ca-ionophore. The LTB4 levels decreased to 3.0 +/- 0.6 ng after 60 min indicating the metabolism of the generated LTB4 by human lung macrophages. This was confirmed by incubation of the cells with exogenously added [3H]LTB4. LTB4 was converted into unpolar products as was identified by thin-layer chromatography and high-performance liquid chromatography; a comparison with the fibroblast cell line L929 which is known to convert LTB4 into the dihydro-LTB4 metabolite (5,12-dihydroxyeicosatrienoic acid) indicates that human lung macrophages use the same pathway of metabolization. Biological inactivation as determined by chemotaxis and cross-reaction with the LTB4 antiserum correlates with the degree of LTB4 metabolism. Moreover, the macrophages convert LTC4 into LTD4 and LTE4 by the enzymatic activity of the gamma-glutamyltranspeptidase and dipeptidase. Our data emphasize that the human alveolar macrophage not only produces arachidonic acid metabolites but modulates the local inflammatory potential by its metabolizing capacity for leukotrienes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-2452477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-2825798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-2889665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-2937643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3003154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3005343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3009610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3012189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3410907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3563417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3740648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3863619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-3924998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-437847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-49201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6088485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6100476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6110427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6122685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6123084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6141997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6267608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6296853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6300870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6320696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6321494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6323538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6326199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-65435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6703469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-6759607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-7119113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-7127909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851540-872868
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
529-36
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2851540-Antibodies, Anti-Idiotypic,
pubmed-meshheading:2851540-Calcimycin,
pubmed-meshheading:2851540-Carcinoma, Bronchogenic,
pubmed-meshheading:2851540-Cells, Cultured,
pubmed-meshheading:2851540-Humans,
pubmed-meshheading:2851540-Immunoglobulin E,
pubmed-meshheading:2851540-Leukotriene B4,
pubmed-meshheading:2851540-Lung,
pubmed-meshheading:2851540-Lung Neoplasms,
pubmed-meshheading:2851540-Macrophages,
pubmed-meshheading:2851540-SRS-A
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pubmed:year |
1988
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pubmed:articleTitle |
Leukotriene generation and metabolism in isolated human lung macrophages.
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pubmed:affiliation |
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, AG Infektabwehrmechanismen, Ruhr Universität Bochum, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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