Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-3-22
pubmed:abstractText
IS1 is one of the smallest transposable elements found in bacteria (768 bp). It contains eight overlapping open-reading-frames (ORFs) greater than 50 codons, designated insA to insG and insB'. To determine which of the ORFs actually code for proteins involved in transposition, we have introduced amber codons into each ORF by site-directed mutagenesis which make neutral changes in the overlapping ORFs. Each mutant IS1 was then tested for its ability to mediate cointegrate formation in Su+ and Su- backgrounds. The mutant elements were also tested for trans-complementation in an IS1-free Salmonella background. Our results show that the products of the insA and insB genes are the only ones essential for cointegrate formation. We suggest that other ORFs may, however, encode accessory proteins.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-2826132, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-2832256, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-2985536, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-3025455, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-3029382, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-3031051, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-3035345, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-383387, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-4605400, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-4894690, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6086942, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6094304, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6100987, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6178738, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6236744, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6237026, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6271475, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6281440, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6307828, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6313938, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6327289, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6327466, http://linkedlifedata.com/resource/pubmed/commentcorrection/2851480-6354260
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
120
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-55
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Mutational analysis of the open reading frames in the transposable element IS1.
pubmed:affiliation
Department of Molecular Biology, University of Southern California, Los Angeles 90089-1340.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't