Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12A
|
| pubmed:dateCreated |
1989-3-6
|
| pubmed:abstractText |
Transcription of proto-oncogene fos is induced by elevated levels of intracellular cAMP. We report that human c-fos promoter recombinants transfected into rat pheochromocytoma cells (PC12) and human choriocarcinoma cells (JEG-3) are induced by stimulation of adenylate cyclase and that this induction is diminished considerably in the mutant PC12 cell line A126-1B2, which is deficient in cAMP-dependent protein kinase II. An element centered at position -60 of the c-fos promoter, which encompasses a consensus cAMP response element (CRE), is sufficient to confer cAMP responsiveness to a herpes thymidine kinase/CAT fusion gene. The specific binding of a nuclear protein to the c-fos CRE can be competed by the somatostatin and alpha-chorionic gonadotropin (alpha-CG) promoter regions that contain CREs. Gel mobility shift assays with double-stranded oligonucleotides containing either the wild-type or mutated c-fos CRE sequence have demonstrated that binding occurs only to the wild-type CRE. The nuclear factor binding to the c-fos CRE is likely to be transcription factor CREB (CRE nuclear binding protein), because an affinity-purified 43-kD CREB isolated from PC12 cells binds efficiently in a DNA footprinting assay. Thus, regulation of the c-fos gene transcription appears to involve a mechanism common to many genes that respond to cAMP as a second message leading to cell growth and differentiation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1529-38
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:2850967-Adenylate Cyclase,
pubmed-meshheading:2850967-Animals,
pubmed-meshheading:2850967-Cell Extracts,
pubmed-meshheading:2850967-Cyclic AMP,
pubmed-meshheading:2850967-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:2850967-DNA-Binding Proteins,
pubmed-meshheading:2850967-Gene Expression Regulation,
pubmed-meshheading:2850967-Humans,
pubmed-meshheading:2850967-Phosphorylation,
pubmed-meshheading:2850967-Plasmids,
pubmed-meshheading:2850967-Promoter Regions, Genetic,
pubmed-meshheading:2850967-Protein Kinases,
pubmed-meshheading:2850967-Proto-Oncogenes,
pubmed-meshheading:2850967-Rats,
pubmed-meshheading:2850967-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:2850967-Transcription, Genetic,
pubmed-meshheading:2850967-Transcription Factors,
pubmed-meshheading:2850967-Transfection,
pubmed-meshheading:2850967-Tumor Cells, Cultured
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Induction of proto-oncogene fos transcription through the adenylate cyclase pathway: characterization of a cAMP-responsive element.
|
| pubmed:affiliation |
Salk Institute, San Diego, California 92138.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|