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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1989-2-23
|
| pubmed:abstractText |
Mutations at the suppressor of Hairy-wing [su(Hw), 3-54.8] locus reverse the phenotype of second-site mutations induced by the gypsy transposable element in Drosophila melanogaster. This gene encodes a protein with a predicted molecular weight of 109,000 that contains an acidic domain and 12 copies of the DNA-binding 'Zn finger' motif. The su(Hw) protein was overexpressed in Escherichia coli and Drosophila cells, and partially purified. It was shown to interact specifically in vitro with a 367-bp DNA fragment that contains 12 copies of the sequence PyPuTTGCATACCPy located in the 5'-untranslated region of gypsy, between the 5' long terminal repeat (LTR) and the first ATG initiation codon. This sequence shows striking homology to some mammalian transcriptional enhancer elements, supporting a role for the su(Hw) protein in the control of gypsy transcription. In addition, the su(Hw) protein is present at approximately 100-200 sites on Drosophila polytene chromosomes, suggesting that it also interacts in vivo with DNA and might be involved functionally in the regulation of normal cellular genes.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1414-23
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2850261-Animals,
pubmed-meshheading:2850261-Base Sequence,
pubmed-meshheading:2850261-Cloning, Molecular,
pubmed-meshheading:2850261-DNA Transposable Elements,
pubmed-meshheading:2850261-DNA-Binding Proteins,
pubmed-meshheading:2850261-Deoxyribonuclease I,
pubmed-meshheading:2850261-Drosophila melanogaster,
pubmed-meshheading:2850261-Enhancer Elements, Genetic,
pubmed-meshheading:2850261-Escherichia coli,
pubmed-meshheading:2850261-Gene Expression Regulation,
pubmed-meshheading:2850261-Immunologic Techniques,
pubmed-meshheading:2850261-Mammals,
pubmed-meshheading:2850261-Molecular Sequence Data,
pubmed-meshheading:2850261-Molecular Weight,
pubmed-meshheading:2850261-Plasmids,
pubmed-meshheading:2850261-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2850261-Suppression, Genetic
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon.
|
| pubmed:affiliation |
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|