Linked Life Data

2848519

Source:http://linkedlifedata.com/resource/pubmed/id/2848519

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-12-30
pubmed:abstractText
A specific receptor for rat atrial natriuretic factor (rANF) was identified on primary cultures of bovine brain capillary endothelial cells (BBCEC's). Cultured BBCEC's have been developed in our laboratory as an in vitro model of the blood-brain barrier. The binding of [125I]-rANF was rapid, reversible, saturable and unaffected by the presence of hormonal peptides such as insulin, vasopressin and angiotensin II. Scatchard analysis of competitive binding studies indicated the presence of a single class of binding sites for rANF with a dissociation constant of 400 pM and maximal binding capacity of 52 fmol/mg total cell protein. Binding of [125I]-rANF was inhibited to varying degrees by atriopeptins I-III with atriopeptin III being the most potent and atriopeptin I being the least potent. BBCEC's also rapidly internalized [125I]-rANF (100% after 60 min at 37 degrees C) by a temperature-dependent process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
308-14
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Characterization of specific receptors for atrial natriuretic factor on cultured bovine brain capillary endothelial cells.
pubmed:affiliation
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, Lawrence 66045.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't