|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
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|
pubmed:dateCreated |
1989-1-4
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|
pubmed:abstractText |
Cell-surface 5'-nucleotidase was assayed by incubating intact cells with 5' [3H]AMP in iso-osmotic buffer and measuring [3H]adenosine production. The activity of cell surface 5'-nucleotidase in small resting lymphocytes and in cells of the B cell line BCL1 was 5.7 and 1.1 nmol/min/mg protein respectively at 37 degrees C. The 5'-nucleotidase was inhibited by Concanavalin A and anti-IgM, the inhibition by anti-IgM being reversible. Incubating the lymphocytes in the presence and absence of mitogens in inositol-free medium for 15 min, 60 min, and 24 h had no effect on 5'-nucleotidase activity. The reaction product adenosine as well as adenine nucleotides were shown to inhibit mitogen-induced proliferation.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0165-2478
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
19
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
71-6
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|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
pubmed-meshheading:2847980-5'-Nucleotidase,
pubmed-meshheading:2847980-Adenine Nucleotides,
pubmed-meshheading:2847980-Adenosine,
pubmed-meshheading:2847980-Animals,
pubmed-meshheading:2847980-Cell Line,
pubmed-meshheading:2847980-Inositol,
pubmed-meshheading:2847980-Lymphocyte Activation,
pubmed-meshheading:2847980-Lymphocytes,
pubmed-meshheading:2847980-Male,
pubmed-meshheading:2847980-Mice,
pubmed-meshheading:2847980-Mice, Inbred BALB C,
pubmed-meshheading:2847980-Mitogens,
pubmed-meshheading:2847980-Nucleotidases
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|
pubmed:year |
1988
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|
pubmed:articleTitle |
Role of inositol starvation on ecto-5'-nucleotidase activity during mitogen-induced lymphocyte activation.
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|
pubmed:affiliation |
Institut für Immunbiologie der Universität Freiburg, F.R.G.
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pubmed:publicationType |
Journal Article,
In Vitro
|
